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oai:drum.lib.umd.edu:1903/309932023-10-13T07:46:04Zcom_1903_11810com_1903_12com_1903_19654com_1903_2col_1903_2749col_1903_19655col_1903_3

Plough, Louis V Fowler, Chelsea Digital Repository at the University of Maryland University of Maryland (College Park, Md.) Marine-Estuarine-Environmental Sciences 2023-10-13T05:32:16Z 2023-10-13T05:32:16Z 2023 https://doi.org/10.13016/dspace/mha3-mw9q http://hdl.handle.net/1903/30993 Environmental DNA (eDNA) tools can address gaps in fish assessment data while reducing the cost and the impact of sampling on threatened anadromous alosine fishes in Chesapeake Bay. Here, I tested the ability of high-frequency eDNA sampling of river herring to predict fish abundances from sonar-based fish counts on the Choptank River and developed and validated novel species-specific eDNA assays for American and hickory shads. River herring eDNA concentrations from daily eDNA sampling were highly correlated to sonar-based fish counts (Spearman’s Rho = 0.84). This relationship informed a model that could accurately predict fish count from eDNA and relevant covariates (R2 = 0.88). The two new shad assays are highly specific and quantitative, and field testing validated detections in Delaware, Maryland, and North Carolina. This work provides a set of eDNA monitoring tools for the Mid-Atlantic alosines and highlights the capacity for eDNA data to generate quantitative metrics of fish abundance. en Investigating the Utility of Environmental DNA Analysis for the Monitoring and Management of Mid-Atlantic Alosine Fishes Thesis Ecology Environmental science Natural resource management anadromous Chesapeake Bay eDNA environmental DNA river herring shad open.access