2026-09-21T04:11:55Zhttps://api.drum.lib.umd.edu/server/oai/request

oai:drum.lib.umd.edu:1903/112782016-03-29T05:21:13Zcom_1903_11811com_1903_12com_1903_2col_1903_2750col_1903_3

Muro, Silvia Serrano, Daniel Digital Repository at the University of Maryland University of Maryland (College Park, Md.) Cell Biology & Molecular Genetics 2011-02-19T07:16:44Z 2011-02-19T07:16:44Z 2010 http://hdl.handle.net/1903/11278 Engagement of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells (ECs) by anti-ICAM coated beads generates vesiculization via cell adhesion molecule (CAM)-mediated endocytosis, a clathrin-/caveolae-independent pathway involving Na+/H+ exchanger 1 (NHE1). ICAM-1 itself plays a key role in transendothelial migration (TEM) of leukocytes, particularly via the transcellular route. This involves endothelial endocytic vesicles that coalesce into transmigration pores, through which leukocytes transmigrate without disrupting EC junctions. The contribution of CAM-mediated endocytosis to the formation of docking sites and vesicular structures supporting TEM was explored in this study. Results show that the ICAM-1/NHE1-dependent CAM-mediated pathway associates with acid sphingomyelinase and ceramide. This supports plasmalemma deformability and cytoskeleton rearrangement, bridging these events to the formation of endothelial docking structures and vesicles involved in leukocyte transmigration. ROLE OF ACID SPHINGOMYELINASE IN ICAM-1/NHE1-DEPENDENT ENDOCYTOSIS: IMPLICATIONS IN LEUKOCYTE TRANSMIGRATION Thesis Cellular Biology acid sphingomyelinase CAM-mediated endocytosis ICAM-1 leukocyte transmigration sphingomyelin/ceramide pathway transcellular open.access