ABSTRACT Title of Proposal: THE EFFECT OF INSPIRATORY AIR HUMIDITY AND TEMPERATURE ON PERFORMANCE TIME WHILE WEARING A RESPIRATOR Master of Science, Erica B. Francis Directed By: Biological Resources Engineering Dr. Arthur Johnson Respirators are worn in only about 20-30% of the appropriate circumstances. This research examined the effect of inspired air conditions on performance time. An environmental chamber supplied air at 27?C, 28?C, 37?C, and 55?C to a powered air- purifying respirator (PAPR) worn by the subject while exercising in a neutral environment. A mathematical model of performance time as a function of the heat index (HI), respirator familiarity (RF), personality type (SN), and minute volume (V e ) indicated that performance time increased with a decrease in HI and V e and with an increase in RF and SN ratio. A model of performance time as function of user acceptability and the heat index indicated that time to reach a level of ?fairly uncomfortable? decreased exponentially from 5.34 minutes to 2.85 minutes with an increase in the heat index. Performance at the heat index conditions may be described by physiological, subjective, and individual characteristics. THE EFFECT OF INSPIRATORY AIR HUMIDITY AND TEMPERATURE ON PERFORMANCE TIME WHILE WEARING A RESPIRATOR By Erica Bicksler Francis Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science 2006 Advisory Committee: Professor Dr. Arthur Johnson, Chair Professor Dr. Gary Felton Professor Dr. Fredrick Wheaton ? Copyright by Erica Bicksler Francis 2006 ii Dedication To JR and Grace iii Acknowledgements I would like to thank Dr. Arthur Johnson, who was not only my advisor, but a wonderful professor and mentor. I would like to thank him for his guidance and more importantly for his patience through my marriage and pregnancy. I am also grateful to my committee members, Dr. Wheaton and Dr. Felton for their guidance and support. Furthermore, I would like to acknowledge and thank Dr. Richard McCuen for helping me with the modeling and statistics portion of this thesis, as well as his assistance in editing. I would like to thank my subjects, and most importantly, Will Scott for helping during testing, guiding me during the entire process, and helping me to work out the kinks of this project. I would also like to thank everyone involved in the human performance lab, including all graduate assistants and interns. Special thanks to Gary Seibel for making sure the environmental chambers were always up and running for me. I would like to thank my husband for supporting me both emotionally and financially during this time, and most importantly, I?d like to thank my new daughter Grace for giving me a reason to finish my degree. iv Table of Contents Dedication..........................................................................................................................iii Acknowledgements............................................................................................................iii List of Symbols................................................................................................................. vii List of Tables ...................................................................................................................viii List of Figures..................................................................................................................... x Chapter 1: Justification ....................................................................................................... 1 Chapter 2: Literature Review.............................................................................................. 3 2.1. Physiological Response to Temperature and Humidity.......................................... 3 2.1.1. Evaporation....................................................................................................... 3 2.1.2. Humidity Effects on Heat Loss........................................................................ 4 2.2. Heat Exchange in Respirators................................................................................. 4 2.3. Effect of Clothing and Respiratory Protection on Heat Exchange ........................ 5 2.4. Temperature and Humidity as Determinants of User Acceptability....................... 7 2.5. Sensitivity of the face in response to temperature and humidity.......................... 11 2.6. Breathing effects due to environmental conditions .............................................. 12 2.7. Heat Stress Indices................................................................................................ 14 2.8. Research Surveys.................................................................................................. 16 Chapter 3: Research Goal and Objectives ........................................................................ 21 Chapter 4: Research Methods ........................................................................................... 22 4.1. Procedures............................................................................................................. 22 4.1.1. Equipment/Apparatus .................................................................................... 22 4.1.2. Environmental Testing Conditions ................................................................ 25 4.1.3. Orientation and Consent ................................................................................ 26 4.1.4. VO 2 max Graded Exercise Test ...................................................................... 26 4.1.5. 65-70% VO 2 max Subject Testing.................................................................. 27 4.2. Method of Statistical Analysis.............................................................................. 30 4.2.1. Outlier Detection............................................................................................ 30 4.2.2. Model Structure ............................................................................................. 31 4.2.3. Calibration...................................................................................................... 32 4.2.4. Sensitivity Analysis ....................................................................................... 33 4.2.5. Assessing Model Prediction Accuracy .......................................................... 34 4.2.6. Sample Size Determination............................................................................ 35 Chapter 5: Research Results and Discussion................................................................... 37 5.1. Subject Demographics and Characteristics........................................................... 37 5.2. Sensitivity Analysis of Heat Index ....................................................................... 38 5.3. Performance Time ? Heat Index Model................................................................ 40 5.4. Sample Graph Explanation ................................................................................... 45 5.4.1. Rectal Temperature vs. Time......................................................................... 45 5.4.2. User Acceptability vs. Time .......................................................................... 46 5.4.3. User Acceptability vs. Mean Facial Skin Temperature ................................. 51 5.4.4. Mean Facial Skin Temperature versus Time ................................................. 55 5.4.5. Rectal Temperature vs. Mean Facial Skin Temperature................................ 56 v 5.5. Analysis of Factors Affecting C 1 .......................................................................... 58 5.5.1. C 1 versus Minute Volume .............................................................................. 58 5.5.2. C 1 versus SN .................................................................................................. 59 5.5.3. C 1 vs. Respirator Familiarity........................................................................... 60 5.6. Collective PT-HI model........................................................................................ 61 5.6.1. Sensitivity Analysis of the PT-HI model (Equation 5-9)............................... 70 Chapter 6: Conclusions.................................................................................................... 72 Chapter 7: Recommendations for Further Research........................................................ 73 7.1. Equipment............................................................................................................. 73 7.2. Subject Effort........................................................................................................ 74 7.3. Research Methods................................................................................................. 76 Appendix 1 (Subject Excel Data) ..................................................................................... 79 Appendix 2 (Mean Facial Skin Temperature Data).......................................................... 81 Appendix 3 (Acceptability Data) ...................................................................................... 87 Appendix 4 (Rectal Temperature Data)............................................................................ 93 Appendix 5 (Performance Time ? Heat Index Model) ..................................................... 99 Appendix 6 (Survey Data) .............................................................................................. 100 Appendix 7 (C 1 graphs) .................................................................................................. 102 Appendix 8 (Research Surveys)...................................................................................... 107 Appendix 9 (Acceptability Scales) ................................................................................. 114 Appendix 10 (IRB 05-0245) ........................................................................................... 117 Bibliography ................................................................................................................... 127 vi List of Symbols A - Acceptability BACS ? Breathing Apparatus Comfort Scale BTPS ? Body Temperature and Pressure Saturated C 1 ? y-intercept C 2 ? x-intercept C 3 ? scale parameter C 4 ? shape parameter CLQ ? Claustrophobia Questionnaire EI ? Extroversion/Introversion parameter ECG ? Electrocardiogram F ? calibration form parameter F i ? factors that influence output variable O FT ? Facial thermal scale HI ? Heat Index n H - normalized half width HRmax? maximal heart rate (beats/min) HSI BH - Heat Stress Index of Belding and Hatch FT ? Facial Thermal Scale IRB ? Institutional Review Board JP ? Judging/Perceiving parameter MBTI- Myers-Briggs Type Inventory n ? sample size O 0 ? value of O at F i OT ? Overall Thermal Scale PAPR ? Powered Air-purifying Respirator PERQ ? Perceived Effort and Reward Questionnaire PT ? Performance time R ? correlation coefficient RF- Respirator Familiarity RH ? Relative Humidity RI ? Reference Index RS ? Relative Strain Index RPE ? Rating of Perceived Exertion R s ? relative sensitivity SCBA ? Self contained breathing apparatus S e ? standard error of the estimate SN ? Sensing/Intuition parameter S y ? standard deviation of the criterion variable TF ? Thinking/Feeling parameter 2/ t - t statistic at designated alpha  - degrees of freedom V e ? Minute ventilation (L/min) V t ? Tidal volume (L/min) VO 2 ? Oxygen consumption (ml/kg) vii VO 2 max - Maximal oxygen consumption (ml/kg) WBGT ? Wet Bulb Globe Temperature (?C) WGT ? Wet Globe Temperature (?C) i Y -i th predicted value of criterion variable i Y - i th measured value of Y YSI ? Yellow Springs International viii List of Tables Table 4-1. Critical Values R c for a sample size of 10??????????....?....................31 Table 5-1. Absolute and Relative Sensitivities for Temperature??????????....?...39 Table 5-2. Absolute and Relative Sensitivities for Relative Humidity????????....?..39 Table 5-3. Model results for the performance time-heat index (PT-HI) model where PT=C 1 e - C2*HI ?????????????????????????????..???????41 Table 5-4. Average of user acceptability (%) for each of the subjects at the four heat index conditions..?????????????????????????????????..47 Table 5-5. Acceptability (%) values computed from Equation 5-8 for 27?C, 28?C, 37?C, and 55?C for a range of mean facial skin temperatures ??????????????????54 Table 5-6. Performance time model results for varying sample sizes??...????????62 Table 5-7. Performance time (min) predictions from Equation 5-9 for values of V e (L/min), SN (ratio), R (number of times respirator worn), and HI (?C).???????????????63 Table 5-8. Predicted performance time values (min) and observed average performance time values (min) for ten subjects..??????????????????????????.69 Table 5-9. Relative sensitivity analysis of the PT-HI model (Equation 5-9)..???????...71 Table A1.1. Subject Demographics???????????????????????...79 Table A1.2. VO 2 max data???????????????????????????..79 Table A1.3. Performance Time data???????????????????????..79 Table A2.1. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 27?C??????????????????????????????..81 Table A2.2. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 28?C??????????????????????????????..82 Table A2.3. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 37?C??????????????????????????????..84 Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 55?C???????????????????????????????????85 Table A3.1. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 27?C????????????????????????????????????87 ix Table A3.2. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 28?C????????????????????????????????????87 Table A3.3. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 37?C????????????????????????????????????88 Table A3.4. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 55?C????????????????????????????????????88 Table A3.5. Acceptability (%)for each heat index (?C) condition over time (min) calculated from Equation 5-5?????????????????????????????????88 Table A3.6. Time (min) to reach acceptability levels (30%, 40%, 50%) at various heat indices calculated from Equation 5-6??????????????????????????..91 Table A4.1. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 27?C????????????????????????????????..93 Table A4.2. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 28?C????????????????????????????????..94 Table A4.3. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 37?C????????????????????????????????..96 TableA4.4. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 55?C???????????????????????????????????97 Table A5.1. Performance time (PT) values (min) for each subject at various heat indices (?C).100 Table A6.1. Perceived Effort and Reward Questionnaire Results???????????...100 Table A6.2. Claustrophobia Questionnaire Results?????????????????..100 Table A6.3. Physical Activity Questionnaire Results????????????????..100 Table A6.4. Respirator User Questionnaire Results. Frequency represents the number of times the respirator had been worn prior to taking part in the study?????????????..101 Table A6.5. MBTI Personality Type Questionnaire Results?????????????...101 x List of Figures Figure 2-1. Diagram of the climate box used by Nielson et al. (1987), and Gwosdow et al. (1989)????????????????????????????..?????.......8 Figure 4-1. Hose connected from one environmental chamber through the exercise chamber to the respirator mask of the subject ?...?????????????????...??....??22 Figure 4-2. Calibration curves of humidity sensor and commercial psychrometer for temperature and humidity????????????????????????????????...24 Figure 5-1. Functions (n=29) for the PT-HI model (Equation 5-3) for all ten subjects...??????????????????????????????????.42 Figure 5-2. Functions (n=29) for the PT-HI model (Equation 5-3) for subjects 001, 145, and 358 including original data points??????????????????????????..43 Figure 5-3. Comparison of Subject 145 (n=29) and Subject 419 (n=29). Model functions and original data points are shown. Subject 145 has a C 1 value of 140.7 and a C 2 value of 0.05516. Subject 419 has a C 1 value of 11.482 and a C 2 value of 0.002157?..??????????...44 Figure 5-4. Average rectal temperature vs. time for all four heat index values. R 2 =0.9884 for HI=27?C, R 2 =0.9773 for HI=28?C, R 2 =0.9949 for HI=37?C, and R 2 =0.9948 for HI=55?C...?..46 Figure 5-5. Average user acceptability (%) vs. time (min) for each of the four heat index conditions.????????????????????????.?????????..48 Figure 5-6. Model functions (n=29) for predicting time to reach unacceptable levels of comfort of the respirator according to the heat index of the environmental condition for acceptabilities of 30, 40, and 50%...?????????????????????.?????????..50 Figure 5-7. Average user acceptability (%) vs. average mean facial skin temperature (?C) for all four heat index values?..??????????????????.?????????..52 Figure 5-8. Plot of average acceptability vs. average mean facial skin temperature using data from Table 5-5 for the four heat index conditions (n=26)...?????.?????????..55 Figure 5-9. Average mean facial skin temperature (?C) vs. time (min) for all four heat index conditions?????????????????????.?????????..56 Figure 5-10. Average rectal temperature vs. average mean facial skin temperature for all four heat index values as well as original data points?????????????????????57 Figure 5-11. C 1 (from Equation 5-3) vs. V e for female and male subjects (n=10)??????59 Figure 5-12. The relationship between the model coefficient C 1 (from Equation 5-3) and SN for all subjects (n=10)??????????????????????????????...60 xi Figure 5-13. The relationship between the model coefficient C 1 (from Equation 5-3) and Respirator Familiarity for all subjects (n=10)????????????????????.61 Figure 5-14. Performance time (minutes) vs. V e (L/min) computed from Equation 5-9???...64 Figure 5-15. Performance time (minutes) vs. the sensing-intuition (SN) parameter computed from Equation 5-9.??????????????????????????????..65 Figure 5-16. Performance time (min) vs. the number of times of the respirator was worn during manual labor prior to the current study computed from Equation 5-9.?????????..?66 Figure 5-17. Performance time (minutes) as a function of the heat index (?C) of the environmental condition computed from Equation 5-9.????????????????67 Figure 5-18. Predicted performance time (n=4) vs. observed performance time (n=4) at the four heat index conditions. Each observed performance time is an average of the performance times for ten subjects????????????????????????????????68 Figure A3.1. Average acceptability (%) vs. time (min) for all heat index conditions. Data taken from Table A3.5???????????????????????????????..91 Figure A7.1. C 1 vs. V e from Table A1.2. for each subject (n=10)???????????..102 Figure A7.2. C 1 vs. Perceived Effort and Reward Questionnaire results from Table A6.1. for each subject (n=10)???????????????????????????????...102 Figure A7.3. C 1 vs. Claustrophobia Questionnaire results from Table A6.2. for each subject (n=10)???????????????????????????????????103 Figure A7.4. C 1 vs. level of physical activity results from Table A6.3. for each subject (n=10)???????????????????????????????????103 Figure A7.5. C 1 vs. Extroversion/Introversion personality characteristic from Table A6.5. for each subject (n=10)?????????????????????????????...104 Figure A7.6. C 1 vs. Sensing/Intuition personality characteristic from Table A6.5. for each subject (n=10)???????????????????????????????????104 Figure A7.7. C 1 vs. Thinking/Feeling personality characteristic from Table A6.5. for each subject (n=10)???????????????????????????????????105 Figure A7.8. C 1 vs. Judging/Perception personality characteristic from Table A6.5. for each subject (n=10)???????????????????????????????...105 Figure A7.9. C 1 vs. Respirator Familiarity from Table A6.4. for each subject (n=10)???...106 1 Chapter 1: Justification Respirators are worn in the workplace in order to protect the individual from toxic airborne substances; however, respirators are worn in only about 20-30% (Harber et al., 1991; Nielsen et al., 1987; Gwosdow et al., 1989; DuBois et al., 1990) of the appropriate circumstances due to several factors, such as ventilation, work limitation, subject discomfort, psychological effects, thermal loading, and cardiovascular changes. The focus of this research study will be on two of these factors: subject discomfort and thermal loading. High facial skin temperature is a major source of discomfort while wearing a respirator and the most common way to alleviate this discomfort is to remove the mask, although most working conditions do not permit this to occur (Johnson et al., 1997). Firefighters, mine workers, toxic chemical disposal crews, and other persons who require the use of respirators at work are subjected to various ambient conditions that elicit a physiological response. This physiological response may impact performance and, ultimately, the quality of work produced. According to several studies, facial skin temperature increases about 1-2?C while wearing a respirator during resting conditions (Johnson et al., 1997; Fox and DuBois, 1993; DuBois et al., 1990). During the stressful situations that require the use of a respirator, these thermal effects are exaggerated, which makes it difficult for the wearer to determine their limitations; hence, performance is affected. Previous studies have focused primarily on the ambient conditions as a predictor of user acceptability. While 2 this information is important, it is necessary to determine how this acceptability correlates to performance time. This study focused on determining a correlation between combinations of humidity and temperature based on the National Weather Service Heat Index (HI) on performance time at a moderate work rate under various simulated inspiratory air temperatures and humidities. Other physiological parameters such as rectal temperature, facial skin temperature, and user acceptability were evaluated under warm environmental conditions. The temperature and humidity conditions necessary for thermal comfort and optimal performance while wearing a respirator were unknown; these relationships will help to facilitate improved respirator design. Respirators have been studied extensively due to the technical features that impair work performance. A model developed to determine the relationship between inspired air conditions and performance time will provide a useful tool for respirator users. This information will enable an employer to determine where optimum performance is achieved under various warm ambient conditions. Employers will know how long an employee can perform safely according to both physiological and psychological factors. Furthermore, manufacturers may consider upgrading respirator equipment by providing the respirator wearer with a supply of fresh air kept at the most favorable ambient conditions, or design the inner surfaces of the respirator such that they absorb heat from the skin. Ultimately, respirator users will benefit due to increased comfort, easier breathing, and improved work efficiency. 3 Chapter 2: Literature Review The following sections detail the known physiological responses to air temperature and humidity levels, as well as the effect of respirators on thermal discomfort and performance. Studies conducted on the effects of ambient conditions on user acceptability are discussed. Work rates discussed are represented as a percentage of VO 2 max, which is defined as an individual?s maximal aerobic capacity. The method for determining VO 2 max is discussed in section 4.1.4. 2.1 Physiological Response to Temperature and Humidity 2.1.1. Evaporation The primary means for heat dissipation during exercise is evaporation via sweat. This process accounts for about 80% of the total heat loss from a person?s body during exercise. Under resting conditions, 20% of the body?s total heat loss is due to evaporation (Wilmore and Costill, 2004). During exercise, the body?s metabolic activity increases, blood flow increases, and the heart rate increases. Consequently, skin temperature increases, creating a positive temperature gradient with the surrounding air. When the surrounding air is unsaturated, evaporation occurs and heat is dissipated from the body. When the surrounding air is warm and humid, problems arise that create a smaller gradient for evaporation to occur, which inhibits adequate heat loss from the body. 4 2.1.2. Humidity Effects on Heat Loss Humidity levels affect evaporation as well as the body?s perception of thermal stress. When the air is humid, the air contains water molecules that decrease its capacity to accept more water due to the decrease in the concentration gradient. Thus, when a person is participating in vigorous activity in humid conditions, evaporation is greatly reduced. Low humidity results in a higher concentration gradient, and evaporation is maximized; however, if sweat is not produced in direct correlation with the evaporation of water from the skin, the skin may dry out. Thermal stress is often times associated with a person?s psychological state, and the discernment of thermal stress may change with a change in the environmental conditions (Wilmore and Costill, 2004). 2.2 Heat Exchange in Respirators Heat exchange at the face occurs through radiation, conduction, convection, and evaporation. While wearing a respirator, radiation, convection, and evaporation are inhibited. High facial skin temperature is a source of discomfort while wearing respiratory protective devices, as reported in several studies. The comfort levels at rest and during exercise are 34.5?C and 31?C, respectively (Fox and DuBois, 1993). Above these facial skin temperatures, the respirator user reported increasing mask discomfort. The rate of radiation is approximately proportional to the temperature difference between the skin and the inner wall of the respirator face piece, though radiation inside the face piece is negligible. Evaporation through the wall of the respirator is minimal, at best. As for conduction and convection, heat is transferred through hot air from the lungs and bronchi to the skin; however, much of this heat is captured inside the mask and is 5 unable to move through the respirator to the environment. Some heat is carried outside the mask with exhaled air (Fox and DuBois, 1993). Although respirators have expiratory valves, expired air does not leave the mask without mixing in the mask and transferring heat to the mask wall under warm environmental conditions. If air left with minimal resistance, little residual heat would be left inside the mask. This is not the case, however, because the expired air does not form a narrow channel through the expiratory valve. The pressure required to open the valve broadens the air stream, re-circulating some of the expired air throughout the mask (Fox and DuBois, 1993). Consequently, heat builds up on the face and the person begins to sweat, which causes discomfort. These effects are exaggerated with increased exercise and heat stress. 2.3. Effect of Clothing and Respiratory Protection on Heat Exchange In order to gain a greater perspective on thermal effects during exercise, several studies were consulted regarding the effects of heat stress while wearing protective clothing. Clothing effectively inhibits performance in hot, humid conditions by limiting heat exchange from the body to the surrounding air. Impermeable clothing inhibits effective cooling from the body so the head becomes a critical site for heat loss (James et al., 1984); however, wearing a respirator limits heat exchange from the face. Consequently, the effect of the clothing and the respirator limit total body heat loss, which increases discomfort, affecting an individual?s work performance. Clothing adds work by increasing the metabolic cost of performance by adding weight and restricting movement. In a study conducted by Nunneley (1989), results showed that clothing inhibited evaporation by creating a humid environment and 6 inducing thermal strain. In a study conducted by Payne et al. (1994), the relationship between heat production and heat dissipation when wearing protective clothing that inhibited evaporation contributed to a rise in mean skin temperature. White et al. (1989) concluded that wearing protective clothing and respirators induced dangerous thermoregulatory stress to the subject at low intensities of exercise in a neutral environment. In general, the combination of protective clothing and respiratory equipment results in a decrement in performance compared to the unencumbered individual. In most industrial settings where respiratory protection must be worn, impermeable clothing may be required as well. The combination of the two causes an increase in heart rate and core body temperature; these effects are often accompanied by a decrease in performance (White et al, 1989;White and Hodous, 1987; James et al., 1984). In a study conducted by White et al. (1989), two ensembles were worn with a Self Contained Breathing Apparatus (SCBA). The first consisted of light work clothing, and the second consisted of a two piece chemical protective suit. Work was performed at low intensity (23% VO 2 max) under three warm environmental conditions (10.6?C, 22.6?C, and 34?C). Overall, it was concluded that in regards to rectal temperature, differences in the clothing ensemble were not significant, but the effect of thermal environment was significant. High rectal temperature (>39.0?C) was a parameter used to assess the subject?s ability to continue work. Once an individual?s rectal temperature exceeded this value, work was terminated. Furthermore, the thermal gradient of rectal temperature minus mean skin temperature was almost reduced to zero while wearing the chemical protective suit under hot conditions. This was determined as a critical factor in an 7 individual?s ability to tolerate heat. In a study conducted by White and Hodous (1987) the use of a SCBA and impermeable clothing resulted in a decrease of exercise tolerance time by as much as 95.6%. Tolerance time is a parameter defined by White et al. (1989) as the time required to achieve one of the following criteria: 1) 90% of maximum heart rate, 2) rectal temperature of 39.0?C, 3) skin temperature equaling or exceeding rectal temperature, or 4) objective or subjective sign of severe discomfort or fatigue (dizziness, nausea, etc.). 2.4 Temperature and Humidity as Determinants of User Acceptability The effects of temperature and humidity as predictors of performance time have not been extensively studied; however, user acceptability of respirators according to these variables has been the topic of several studies, some of which are discussed below. Nielsen et al. (1987) studied six subjects, all dressed similarly, in various ambient air temperatures, mask air temperatures, and air humidities inside the mask to determine user acceptability during exercise. The subjects exercised for 15 minutes on a cycle ergometer at different combinations of ambient air temperatures (7?C, 16?C, 25?C), mask air temperatures (22?C, 27?C, 33?C), and mask air humidities (61% and 86%). Skin temperature, heart rate, and skin wettedness were monitored during testing. Thermocouples were placed on the skin of the upper lip and on the cheek, and electrocardiogram (ECG) electrodes were fixed to the chest to monitor heart rate. User rating scales were used to assess acceptability of the mask and whole body conditions. The results of the study found that a significant interaction existed between the combined lip temperature and mask acceptability. A significant interaction also existed between mean facial skin temperature and mask acceptability. A high facial skin 8 temperature decreased the acceptability of the mask. Furthermore, low and high (22?C and 33?C, respectively) mask air temperatures resulted in low acceptability, whereas moderate mask air temperatures (27?C and 30?C) were acceptable. Mask acceptability was considerably lower when the mask air was warm and humid than when it was warm and dry. As expected, a higher mask air temperature resulted in a higher lip temperature when the mask air was humid compared to being dry. Gwosdow et al. (1989) reported the effects of thermal discomfort on acceptability of respirators at rest under different combinations of ambient air temperatures (25?C, 30?C, 35?C), mask air temperatures (27?C, 30?C, 33?C), and mask air humidities (47% and 73%) via use of the climate box shown in Figure 2-1. A half- mask respirator was used that covered the mouth, nose, and part of the cheeks. The skin temperature was recorded with thermocouples on the forehead, upper lip, and cheek. Ratings of discomfort, thermal sensation, acceptability, and difficulty breathing were recorded during testing. The results of the study indicated that the face, in particular the cheeks and forehead, are the regions most sensitive to warm stimuli. The relationships between acceptability, discomfort, and thermal sensation versus lip temperature were the basis for this study. According to the results, respirator discomfort and thermal sensation of the Figure 2-1. Diagram of the climate box used by Nielson et al. (1987), and Gwosdow et al. (1989). 9 face increased with an increase in lip temperature. Furthermore, acceptability decreased with an increase in lip temperature, especially above a lip skin temperature of 34.5?C. The acceptability of a respirator was dependent on mask air temperature and humidity. Air temperatures of 27?C and 30?C with either of the two humidity conditions were always 100% acceptable regardless of room conditions. At room temperatures of 25?C and 30?C, the acceptability of the respirator decreased for a mask air temperature of 33?C or above. Similarly, with 73% humidity, a mask air temperature of 33?C or above had a lower acceptability than with 47% humidity. Subjective responses of respirator thermal sensation, discomfort, and acceptability correlated (p<0.05) to lip skin temperature. DuBois et al. (1990) reported results similar to the previous studies mentioned. The study measured facial discomfort as a function of facial skin temperature while using respirators that utilized tidal breathing rather than continuous airflow. In respirators that utilize tidal breathing, heat and water vapor delivered by expired air contribute to the sense of increased temperature and humidity of the face. Skin temperature was recorded via a thermocouple attached to the left nasolabial fold inside the mask, and a rating scale was used to record subject discomfort levels. In this study, the subjects remained at rest. The results of the study indicated that skin temperature of the face increased within a few minutes of putting on the mask. Furthermore, discomfort was correlated with an increase in skin temperature. As the skin temperature increased by 2.0?C, discomfort increased 1 unit. Similar to the previous study, Fox and DuBois (1993) studied the effect of evaporative cooling of respirators on skin temperature and overall thermal sensation and comfort. Resting subjects with lip skin temperatures of 34?C or less reported that the 10 mask conditions were comfortable and almost 100% acceptable. The study by Fox and DuBois (1993) reported results indicating that a high facial skin temperature (above 31?C) is a major source of discomfort. Two masks were used by Fox and DuBois (1993): an aluminum mask with inspiratory and expiratory valves and a modified Scott model 66 twin-cartridge respirator. The facial skin temperature was measured with a thermocouple attached to the nasolabial fold inside the mask. The subjects were measured at rest and during submaximal exercise. The major results from this study indicated that the threshold for the comfort zone of skin temperature inside the mask for resting subjects began at a lip temperature of 34.5?C, and at about 31?C for exercising subjects. Laird et al. (2002) considered the effects of wearing a respirator on heart rate, and facial skin temperature on user acceptability. The study conducted was two-fold: a laboratory study and a workplace study. In the laboratory study, a standard filter respirator was worn for the first 15 minutes of exercise in the first session, and in the second session, the respirator was worn for only the second half of the exercise. The respirator covered the mouth and nose only, and the facial skin temperature was recorded via bead thermistors positioned on the cheek and upper lip. In the workplace study, subjects were asked to simulate their work tasks without a respirator, and then asked to carry out the tasks while wearing the respirator. In the laboratory study, the mean temperature of the lip decreased when the respirator was removed and increased when the respirator was put on. Wearing the respirator did not have a significant effect on heart rate or on the temperature of the cheek. 11 Selkirk et al. (2004) recently studied the limits of firefighters in warm and humid environments. The relationship between tolerance time to the work while wearing a respirator and work rate at three different environmental temperatures (25?C, 30?C, 35?C at 50% humidity) while wearing protective clothing was the main focus of the study. The results showed that tolerance time decreased in response to an increase in temperature regardless of the work rate. 2.5. Sensitivity of the face in response to temperature and humidity In many industrial situations, workers are required to wear protective clothing, which effectively inhibits evaporation from the body, so the head becomes the primary outlet for heat escape. In many situations where protective clothing is required, a respirator may also be required. In this case, evaporation from the face is repressed, forcing heat to build up in the body. White et al (1991) indicated that the most common cause of discomfort while wearing respiratory protection was excessive heat inside the devices. According to Gwosdow et al. (1989), these thermal conditions may often lead to subjective fatigue and an increase in the number of mental errors. Preliminary research in our lab indicates that out of 165 surveyed respirator users, more than 40% indicated the most common reason for removing the respirator or not wearing it at all is that the respirator caused them to become too hot and sweaty (unpublished results). The heat flux per unit area of the bare face, 104 W/m 2 , is double that of the rest of the body, which is about 50 W/m 2 (DuBois et al., 1990). While wearing a respirator, the heat flux diminishes, causing an increase in temperature of the skin under the mask (DuBois et al., 1990). Whereas the mechanism for discomfort is unknown, it is acknowledged to be a function of facial skin temperature, and may be caused by thermal 12 sensation, sweating and hydration, condensation of expired air, or cutaneous blood flow (Gwosdow et al., 1989; DuBois et al, 1990; Nielsen et al., 1987). Under normal conditions, the whole body thermal sensation is proportional to the area-weighted mean skin temperature; the findings by Gwosdow et al (1989) indicated that this relationship changed when thermal conditions inside the respirator changed. For example, at a room air temperature considered neutral (25?C), increasing the respirator thermal conditions (temperature and humidity) changed the whole body thermal sensation from neutral to warm. Nielsen et al. (1987) reported similar findings. According to Nielsen et al. (1987), the use of different air temperatures in the mask compared to the ambient air produces local thermal stimuli to the skin surface beneath the mask, changing both the heat exchange from the skin surface to the air inside the mask and the heat exchange from the skin area under the edges of the mask. 2.6. Breathing effects due to environmental conditions In a study conducted by Louhevaara et al. (1984), the effects of three different respirators on pulmonary ventilation, oxygen consumption, and heart rate were examined during rest, submaximal work, and recovery in well-trained young healthy men. The conclusions of the study indicated that the dead space within the respirator increased the concentration of carbon dioxide in the inspired air, stimulating an additional effort in breathing, which subsequently increased oxygen consumption and heart rate. However, according to Johnson et al. (2003), air-purifying respirator use under a variety of work conditions did not stress the cardiovascular system. Thus, this phenomenon can be readily disputed. 13 In the studies discussed in the previous section conducted by Nielsen et al. (1987) and Gwosdow et al. (1989), the results indicated that local thermal strain interfered with respiratory heat exchange. The perceived work of breathing became more difficult as the heat content of the inspired air increased. This was the case for only the conditions in which a mask was worn, and breathing was most difficult in the condition where the mask air was humid. Lekeux (1988) studied the effect of environmental conditions on the breathing pattern of ponies. The ambient temperature and humidity varied only according to the daily environmental conditions. Temperature and humidity were recorded prior to exercise and combined together to form a unitless measurement of the environmental condition. Measurements less than 85 indicated cold and dry conditions, while measurements greater than 85 indicated warm and humid conditions. The results of the study indicated that the conditions did increase the frequency of breathing. Turner et al. (1992) performed a similar experiment on human subjects. The results of Lekeux (1988) indicated that the frequency of breathing decreased in the cool, dry conditions, and tidal volume increased in the warm, humid conditions. The increase in tidal volume likely contributed to an increase in minute ventilation. Johnson et al. (2005) performed a study to determine peak inhalation flow rates during strenuous exercise. Flow rates were measured at 80-85% VO 2 max without a respirator, with a powered air-purifying respirator (PAPR) and at the conclusion of the VO 2 max test without a respirator. Major conclusions of the study indicated peak flow rates of up to 359 L/min (BTPS) for both respirator conditions, with flow rates for the 14 PAPR exceeding breathing flow rates. Furthermore, peak flow rates of up to 579 L/min (BTPS) were observed at 100% VO 2 max. 2.7. Heat Stress Indices Several attempts have been made at trying to find heat stress indices that predict the effects of hot environments on human physiological conditions and performance. There are over a dozen heat stress indices available; however many of them utilize heat loss, sweat loss, rectal temperature, and other physiological parameters in their calculation. For the purposes of this study, a heat stress index is required in order to relate temperature and humidity. One of the most widely used is known as the wet bulb- globe temperature (WBGT) index because it incorporates the effect of temperature, humidity, radiation, and air movement. McCann and Adams (1997) correlated WBGT index with performance in competitive distance runners. Their results indicated that optimal conditions, designated by the best performance times were difficult to predict with a linear model. Instead, they found that a curvilinear relationship more accurately described the relationship between several combinations of hot and humid environments and physiological responses. Their major results showed that, regardless of how high the WBGT index was, runners performed better at lower humidity levels than at higher humidity levels. Thus, the same WBGT index may produce different results in performance if in one case, temperature is high and humidity is low, and in the other, if temperature is lower but humidity is high. Klemm and Hall (1972) delved into the issue of the utility of heat stress indices on physiological strain. In their study, 20 different combinations of dry bulb-wet bulb 15 temperatures were selected. The participants remained at rest as physiological measurements were recorded. Their results indicated that indices with lower dry bulb temperatures and higher wet bulb temperatures induced lower strains than those with higher dry bulb temperatures and lower wet bulb temperatures. The conclusion, then, was that temperature and humidity differently affected the physiological response of the individual. Ramanathan and Belding (1973) performed a study to determine the utility of the WBGT. The objective of their study was to evaluate the WBGT index under combinations of environmental conditions and work rate. Their results proved that a given level of WBGT had meaning dependent on environmental conditions, but that higher levels on the WBGT scale do not necessarily signify greater strain than lower levels. Pulket et al. (1980) performed a study to compare available heat stress indices in a hot-humid environment. His results indicated that the wet globe temperature (WGT) index gave the best correlation with physiological strain related to heat loss through the skin. The WGT index is a single reading of the Botsford wet globe thermometer that exchanges heat with the surroundings by convection, evaporation, and radiation in a manner similar to that of a sweating man. However, the WGT may not be completely suitable as a field heat stress index, but it did give a higher correlation with physiological strain than did the WBGT index. Several other indices were studied, including the Relative Strain (RS) index, Reference Index (RI), and the Heat Stress Index of Belding and Hatch (HSI BH ). These all correlated with composite physiological strain; however, 16 these heat stress indices incorporated heat loss, clothing effects, and other non- environmental factors. In order to evaluate the physiological strain of an individual with the environment, it is necessary to determine a heat index that effectively combines the effects of temperature and humidity. The National Weather Service (2005) has developed a heat index equation as a result of extensive biometeorological studies. The index is a measure of how hot it feels when relative humidity (RH) is added to the actual air temperature. However, the equation is only useful for temperatures of 27?C (80?F) or higher, and relative humidites of 40% or greater. 2.8. Research Surveys One of the objectives of this research was to model work performance time. This includes fitting coefficients of the model and explaining the factors that influence the values of the coefficients. Therefore, several surveys were administered to each of the subjects in order to explain why some of the subjects had a good fit with the performance time model while others did not exhibit a good fit. Five surveys were administered to each individual at the completion of their four testing sessions: Perceived Effort and Reward Questionnaire (PERQ) (Tremblay et al., 2002), Claustrophobia Questionnaire (CLQ) (Radomsky et al., 2001), Raffenbarger Physical Activity Questionnaire (Raffenbarger et al.,1978), Respirator User Questionnaire (unpublished research), and the Myers-Briggs Type Inventory (MBTI) (Culp et al., 2001). All surveys are located in Appendix 8. 17 The respirator could be perceived by some as a constraint, specifically, one that is claustrophobic. The CLQ was selected to measure this aspect of the test. The CLQ is a quantitative measure of a person?s tendency to feel claustrophobic in certain situations. Claustrophobia is the fear of enclosed spaces and can often be unpleasant for people who are unable to cope with the fear of what might happen in that enclosed space. Claustrophobia is a combination of two separate fears: fear of suffocation and fear of restriction. The CLQ consists of 14 questions that pertain to fear of suffocation and 12 questions that pertain to fear of restriction. The test demonstrates high test-retest reliability, consistency, good discriminant validity, and predictive validity (Radomsky et al., 2001). Because of its strong predictive and discriminant validity, the CLQ may be used in a variety of research applications. The coefficients of the model may depend on the physical conditioning of the subject. Therefore, a test was selected to provide a measure of the subject?s usual level of physical activity. The Raffenbarger Physical Activity Questionnaire was developed by Dr. Ralph Raffenbarger Jr. for disease epidemiology studies. The questionnaire estimates the number of kilocalories people expend per week in both sports and leisure activities. The survey is easy to complete and consists of questions that pertain to the number of stairs climbed per day, number of city blocks walked per day, and any sports or activities performed, including frequency and duration. The subject is asked to average their activity over the entire year, and the researcher performs simple calculations to convert the activity to total energy expenditure. A person?s performance can be influenced by their familiarity with the equipment. Therefore, an index of mask familiarity was selected. The Respirator User Questionnaire 18 was developed by Dr. Arthur T. Johnson (unpublished research). The questionnaire evaluates a person?s familiarity with the respirator, reasons for discomfort while wearing the respirator, their overall attitude toward respirator use, and their ability to perform work while wearing the respirator. The questionnaire was shortened to contain only questions relevant to respirator use during the current study and was used to assess the individual?s familiarity with the respirator, as well as their overall attitude toward the respirator. The MBTI has been used for over 60 years and over this time, it has become one of the most widely used psychological instruments. According to Consulting Psychologist Press, Inc. (Mountain View, CA), the MBTI is completed by approximately 2 million people per year. Many schools and employers use the instrument as a means for profiling characteristics associated with different personality types. These personality types have been characterized (Culp et al., 2001) by four scales with opposite poles: extraversion (E)-introversion (I), sensing (S)-intuition(N), thinking(T)-feeling(F), and judging(J)-perceiving(P) Based on these scales, 16 distinct personality types emerge. The tendencies of each scale (SN,TF, JP, and EI) were represented by a fraction in order to reduce the number of predictor variables within the MBTI parameters. The following equations represent each grouped variable (Koh, 2004):  EI = number of Extraversion positives ? Total number of questions for Extraversion-Introversion  SN = number of Sensing positives ? Total number of questions of Sensing- Intuition. 19  TF = number of Thinking positives ? Total number of questions for Thinking- Feeling.  JP = number of Judging positives ? Total number of questions for Judging- Perceiving. A person who is extraverted (E) acts first and thinks later, feels deprived when cutoff from interaction with the outside world, is usually open to and motivated by people, and enjoys a wide variety of relationships. A person who is introverted (I) thinks first then acts, requires private time to recharge, is motivated internally, and prefers one- on-one communication. A person who exhibits sensing (S) characteristics mentally lives in the present, uses common sense and creates practical solutions by instinct, has rich memory recall, and likes clear and concrete information. An intuitive (N) person mentally lives in the future, uses their imagination and creates new possibilities by instinct, improvises best through theoretical understanding, and is comfortable with ambiguous, fuzzy data. A person who exhibits thinking (T) characteristics instinctively searches for facts and logic in a decision situation, naturally notices work that must be accomplished, is able to provide an objective analysis easily, and accepts conflict as a natural part of relationships. A person who exhibits feeling (F) characteristics instinctively employs personal feelings in decision situations, is naturally sensitive to people?s needs, naturally seeks popular opinions, and is unsettled by conflict. A person who exhibits judging (J) characteristics plans in advance before taking action, is focused on task-related action, works best when keeping ahead of deadlines, and naturally uses targets and standard routines to manage life. A person who exhibits 20 perceiving (P) characteristics is comfortable moving into action without a plan, likes to multitask, is naturally tolerant of time pressure and works best close to deadlines, and instinctively avoids commitments that interfere with flexibility and freedom. Some subjects were able to tolerate the hot, humid conditions better than others. The physiological and psychological factors inferred from the surveys were used to explain variations in performance time with the environmental conditions. 21 Chapter 3: Research Goal and Objectives The goal of the proposed research project is to develop a mathematical model that correlates performance time with the National Weather Service Heat Index (HI) to provide manufacturers with a useful tool for designing masks with the most favorable characteristics that are appropriate for optimal performance at the environmental conditions (i.e. temperature and humidity) prevalent at the location of use. To meet this goal, this research will address the following three objectives: Model the relationship between inspired air conditions (humidity and temperature) and performance time while wearing a respirator; Evaluate the user acceptability parameter (Breathing Apparatus Comfort Scale, or BACS) and assess its relationship with facial skin temperature; Model the relationship between performance time with several parameters that may serve as predictors of performance time at the four environmental conditions - Correlate performance time with rectal temperature, facial skin temperature, and user acceptability - Examine factors that may pre-determine an individual?s performance time for screening purposes 22 Chapter 4: Research Methods This research consisted of three stages: obtain subject consent and orientation, perform the VO 2 max graded exercise test, and complete 65-70% VO 2 max testing under three different conditions. A total of 10 subjects were recruited for the study. 4.1 Procedures 4.1.1. Equipment/Apparatus 4.1.1.1. Environmental Chambers In order to supply warm humid air to the respirator mask, two Kolpak environmental growth chambers (Integrated Development & Manufacturing Company Chagrin Falls, OH) were used. One environmental chamber was maintained at 30?C and 40% humidity. The subject exercised in this chamber, while air from the other environmental chamber was drawn into the exercise chamber via a long hose that was connected to the respirator mask (Figure 4-1). The temperature and humidity in this chamber changed for each testing session, whereas the temperature and humidity in the chamber used for exercise remained constant. Figure 4-1. Hose connected from one environmental chamber through the exercise chamber to the respirator mask of the subject. 23 The walk-in chambers are modular and consist of several aluminum tongue and groove sectional panels that contain four inches of foamed in-place polyurethane for insulation. Each chamber consists of temperature control, a humidifying system, and a dehumidifying system. A digital display on the exterior of each chamber allows for programmed control of these systems. The temperature control ranges from 1.7?C to 46.1?C (35?F to 115?F) and the humidity control ranges from 0% to 99%. 4.1.1.2. Temperature/Humidity Sensor The Taylor Series 1452/1455 temperature and humidity sensor (Speranza?s Weather House; Hendersonville, NC) used for the research consists of an LCD display and external sensor probe with a 10 foot fixed cable. The sensor measures indoor temperatures of -5?C to 50?C (23?F to 122?F) and outdoor temperatures of -50?C to 70?C (-58?F to 158?F), and measures indoor humidity from 20% to 99%. The resolution is 0.1 degrees F/C and 1% for humidity. Although the resolution is good and the calibration with a sling psychrometer (Belfort Instrument Company Baltimore, MD) is sufficient, the reaction time for displaying a change in humidity is relatively poor. The indoor-outdoor temperature and humidity sensor was calibrated using a sling psychrometer (Belfort Instrument Company Baltimore, MD) at temperatures ranging from 62?F to 93?F and humidity ranging from 44% RH to 78% RH. The sensitivity of the apparatus was relatively low, requiring an average time of 2 minutes to accurately display the relative humidity. An analysis of variance performed indicated no significant difference (p < 0.05) between the actual temperature and humidity recorded by the psychrometer and the temperature and humidity displayed on the sensor. The R 2 24 Figure 4-2. Calibration curves of humidity sensor and sling psychrometer for temperature and humidity (n=7). value for the temperature calibration was 0.99 and the R 2 value for the humidity calibration was 0.97 as seen in Figures 4-2 above. 4.1.1.3. Powered Air Purifying Respirator (PAPR) with blower The respirator mask chosen for this research was a Breathe Easy Turbo tight- fitting powered air purifying respirator (3M St. Paul, MN) with a belt-mounted blower and filtration unit; however the filtration was excluded from the study. The complete system used in the research included a blower unit, respirator headpiece, breathing tube, and a 4.5V external power source. Johnson et al. (2005) published voltages as well as the corresponding flow rates produced, and percentages of the maximum flow rate while wearing the tight fitting powered air purifying respirator (PAPR). Based on this research, at 4.5V supplied to the motorized blower, the average flow rate produced is 103.46 L/min, which is 94% of the maximum flow rate able to be sustained by the blower unit. Temperature Calibration y = 0.9346x + 4.5352 R 2 = 0.9966 0 10 20 30 40 50 60 70 80 90 100 50 55 60 65 70 75 80 85 90 95 100 Sensor (?F) P s y c h r o m e t e r ( ? F ) Humidity Calibration y = 1.0555x - 3.5747 R 2 = 0.9756 0 10 20 30 40 50 60 70 80 90 30 40 50 60 70 80 90 Sensor (% RH) P s y c h r o m e t e r ( % R H ) 25 4.1.2. Environmental Testing Conditions During each testing session, the subject exercised in an environmental chamber that remained at 30?C and 40% humidity. The respirator was supplied with the following four combinations of temperature and humidity: 27?C, 50% humidity 27?C, 70% humidity 32?C, 60% humidity 37?C, 70% humidity In order to compare the conditions according to one variable, each state was assessed by the heat index developed by the National Weather Service. Regardless of the magnitude of the individual environmental factors, the physiological responses will likely be different at different heat index values. Choice of the conditions was based upon the heat index to see if there is a difference in performance time as the conditions feel warmer. The following equation calculates HI based on two environmental variables and was developed for temperatures greater than 27?C (80?F) and relative humidity greater than 40% (National Weather Service, 2005): HI = -42.379 + 2.04901523T + 10.14333127RH ? 0.22475541TRH ? 6.83783x10 -3 T 2 ? 5.481717x10 -2 RH 2 + 1.22874x10 -3 T 2 RH + 8.5282x10 -4 TRH 2 ? 1.99x10 -6 T 2 RH 2 (4-1) where HI = Heat Index (?F); T = ambient dry bulb temperature (?F); RH = relative humidity (integer percentage). All heat index values were converted to ?C. 26 According to the above equation, each of the testing conditions may be converted to following heat index: At 27?C, 50% humidity, HI = 27?C At 27?C, 70% humidity, HI = 28?C At 32?C, 60% humidity, HI = 37?C At 37?C, 70% humidity, HI = 55?C 4.1.3. Orientation and Consent Institutional Review Board (IRB) approval on IRB application #05-0245 was received on May 18, 2005 (Appendix 10). Ten young, healthy, normally conditioned subjects were recruited for the study. Of these subjects, approximately half were not familiar with wearing the respirator; therefore, each subject was fully advised as to the requirements of their participation. The subjects read and signed the informed consent document and medical history questionnaire. The orientation session provided the subject with a detailed description of their rights, and it provided the investigators with information about the subjects? health and ability to partake in vigorous activity. Any demographic or experimental data collected corresponded only to a subject number and may not be traced back to the individual. 4.1.4. VO 2 max Graded Exercise Test All prospective participants performed a maximal oxygen consumption test on a motorized treadmill (model 15.0Q, Image, Logan, UT) in order to determine each subject?s maximal aerobic capacity. This test was used to develop each subject?s work rate during each of the testing sessions and each subject?s critical end point values. 27 Participants warmed-up and stretched for approximately ten minutes prior to the start of the test. The mask used during the test was a half-mask equipped with one-way inhalation and exhalation valves (Hans Rudolph Inc., Kansas City, MO). This apparatus was interfaced with a standard Fleisch pneumotach (Phipps & Bird, Richmond, VA) and mass spectrometer (Perkin-Elmer, Pomona, CA) to monitor continuous expired airflow. Heart rate measurements were assessed using a Polar S810i wireless heart rate monitor (Polar Electro Inc. Lake Success, NY). In order to determine VO 2 max, the subject participated in a graded exercise test to exhaustion. The work rate was adjusted every three minutes until the participant became fatigued, failed to display a rise in oxygen consumption in accordance with the increase in work rate, or reached a maximal heart rate as determined from the following equation (Johnson, 2004): ageHR = 220 max (4-8) where HR max =maximum heart rate (beats/min). All subjects? VO 2 max data may be found in Table A1.2. in Appendix 1. 4.1.5. 65-70% VO 2 max Subject Testing Each session was conducted at 65-70% of the participant?s maximal oxygen consumption using the motorized treadmill. All sessions utilized a tight-fitting powered air purifying respirator (PAPR), which covered the entire face including the cheeks and forehead and supplied air to the respirator via a motorized blower. The advantage to using the PAPR is to ensure a constant flow of the humid air to the user. The subject exercised at a constant work rate throughout all four sessions. Furthermore, each of the subjects dressed similarly, wore the same mask throughout all sessions, and exercised at 28 the same temperature and humidity within an environmental chamber. The only changing variables were temperature and humidity within the respirator. Testing conditions were randomized for each subject prior to the commencement of the study. Before exercise, the subject completed a five minute warm-up on the treadmill followed by five minutes of stretching. The subject donned the heart rate monitor, a rectal probe (YSI 423, Yellow Springs International, Dayton, OH) was self-inserted 10 cm inside the sphincter for measuring core body temperature, and three surface temperature sensors (YSI 4400, Yellow Springs International, Dayton, OH) on the face. The accuracy of the YSI 423 rectal probe was ?0.2?C from -1 to 60?C, ?0.1?C from 25 to 45?C and the accuracy of the YSI 4400 temperature sensors was ?0.2?C for a range of -41? to +105?C. A Yellow Springs International (YSI) Precision 4400 Series telethermometer was connected to the sensors to record instantaneous skin temperature measurements (Yellow Springs, OH). The sensors were placed on the mid-forehead, right cheekbone, and upper lip under the left nostril. Mean skin temperature was calculated based on an equation proposed by Gwosdow et al (1989) for a weighted mean of ten local skin temperatures. The weightings for the forehead, cheek, and upper lip were 0.046, 0.012, and 0.012 respectively. Dividing each by the sum of the three yields the following equation for mean skin temperature: )(171.0657.0 lchfoface TTTT ++ = (4-9) where T face is the surface area weighted mean skin temperature for the face in ?C, T fo is the forehead sensor temperature in ?C, and T ch is the cheek sensor temperature in ?C, and T l is the upper lip sensor temperature in ?C. 29 After the subject donned the heart rate monitor, skin temperature sensors, and inserted the rectal probe, the subject dressed in standard military fatigues and tennis shoes. A full face piece respirator was fitted to the subject for a comfortable, but snug fit. The inlet of the respirator was connected via a breathing hose to the motorized blower that supplied air to the user from an environmental chamber. At the outlet of the respirator, a combined temperature/humidity sensor probe monitored temperature and humidity of the expired air. The subject began to exercise at a treadmill speed and grade set at a work rate below 65-70% VO 2 max; the speed and grade were increased for approximately 90 seconds before the final speed and grade corresponding to 65-70% VO 2 max was reached. At this work rate, the subject exercised until he or she reached exhaustion. While the measure of exhaustion is purely subjective, the human monitor during subject testing observed whether substantial effort had been achieved based on maximum heart rate, Rating of Perceived Exertion (RPE), or whether the subject expressed signs of severe discomfort. The same human monitor was present throughout each of the testing sessions. After each testing session, the subject was given a 5 minute cool-down then asked to provide reasons for termination. Four scales were used to determine how the subject felt every two minutes. The Rating of Perceived Exertion (RPE) scale was used to determine how difficult the subject felt the work was. A low score of 6 indicated that the work was very, very light, and a high score of 20 indicated that the work was very, very hard. The Breathing Apparatus Comfort Scale (BACS) was used to determine how comfortable the subject felt the respirator was. A low score of 0 indicated that the mask was very, very uncomfortable, 30 and a high score of 10 indicated that the mask was very, very comfortable. The Facial Thermal (FT) scale was used to determine how warm the subject?s face felt. A low score of 1 indicated that the subject?s face felt very cold, and a high score of 7 indicated that the subject?s face felt very hot. The Overall Thermal (OT) scale was used to determine how warm the subject?s overall body felt. This scale is identical to the FT scale. All scales are found in Appendix 9. Temperatures and humidities of both chambers were recorded prior to each testing session and following each testing session. Heart rate was recorded every 5 seconds and downloaded to a computer using the Polar Precision Performance software. Facial skin temperatures and core body temperatures were recorded every 30 seconds. Chamber temperature and humidity, the RPE, BACS, FT, and OT were recorded every two minutes. For the model, the dependent variable is performance time, indicated by the amount of time the subject was able to exercise until he or she reached exhaustion. Performance time was modeled as a function of the HI to determine the effect of the respirator. Since performance time was an individual subject?s termination time, it was very subjective. Therefore, several physiological and psychological factors may explain the variability of performance time. 4.2 Method of Statistical Analysis 4.2.1. Outlier Detection The Dixon-Thompson outlier test is used to statistically decide if an extreme event can be considered as an outlier (McCuen, 2003). The test is applicable for sample 31 sizes as small as three. In order to conduct the test, the data are ranked from smallest (X 1 ) to largest (X n ), and the test statistic R is computed according to the sample size. For a sample size of 8 to 10, the following equation for R is used:    =  2 1 XX XX R n nn (4-10) where X n-1 is the next to largest sample value, X 2 is the next to smallest value. The following are critical values (per % level of significance) for a sample size of 10: Table 4-1. Critical Values R c for a sample size of 10 Critical Value 5% 2.50% 1% 0.472 0.528 0.59 If the computed R is greater than R c , then the null hypothesis that the data point is not an outlier is rejected, and the data point (X n ) is considered to be an outlier. 4.2.2. Model Structure 4.2.2.1.Graphical Analysis Graphical analyses are a useful first step in modeling to understand the structure of data. Graphical analyses provide information on the effects of the independent variables on dependent variables. For the proposed research, an association was expected between performance time and the HI. Similarly, a correlation was expected between facial skin temperature and acceptability. This part of the modeling effort provided a qualitative assessment of the degree to which one variable may be used to predict another variable (McCuen, 1985). Graphical analyses are useful for identifying possible extreme events (outliers), the degree of association between variables, and the form of the 32 relationships. Misrepresenting the form of a relationship can reduce the accuracy of predictions made based on a model developed from the data. Data may take several forms, each of which may be affected by the occurrence of extreme events, or outliers. The first step is to identify the presence of outliers, then to identify the form and type of relationship between the variables (McCuen, 1985). If extreme events occurred, these data points may affect the general trend of the data. The data may take a linear or nonlinear form and have different degrees of correlation. The data may be positively or negatively correlated, and the degree of correlation differs depending on the shape, trend, and slope of the data. Graphs aid in the selection of the model structure that most accurately represents the data and the physical processes being modeled. 4.2.3. Calibration Linear models do not always fit data to an acceptable degree; additionally, they may provide irrational predictions when the intercept is negative. Thus, numerical optimization is a method of fitting nonlinear functions. Calibration may occur via analytical, numerical, or subjective optimization. Once the graphical analyses have been performed and a model structure identified, calibration is a process that determines the coefficients by minimizing the sum of the squares of the errors. This equation takes the following form:  = = n i i i YYF 1 2 )(min (4-11) where i Y is the i th predicted value of the criterion variable, i Y is the i th measured value of Y, and n is the number of observations on the criterion variable. Analytical optimization 33 utilizes differential equations to derive the unknown coefficients from objective functions. For example, the derivative of Equation 4-11 with respect to the unknowns is set equal to zero, and the coefficients are determined by solving a set of simultaneous equations. For numerical optimization, on the other hand, the coefficients are determined using an iterative, but systematic process. The advantage to using the numerical process as opposed to the analytical process is that it can be used with more complex model structures and for non-differentiable model forms. 4.2.4. Sensitivity Analysis A model is intended to reflect the physical processes from which the data were measured. A sensitivity analysis is a useful tool for assessing whether or not the model is a rational reflection of the processes. In order for the model developed to be applicable to determining responses on a larger scale, the sensitivity of the model should be analyzed. In other words, how sensitive the model is to errors, how important each of the variables and coefficients are in affecting the output, and how sensitive the goodness of fit is. The general definition of sensitivity can be described as a function that reflects the effects of inputs on the output of the model. Differentiating the output variable with respect to each factor and including system responses yields the general equation for the relative sensitivity of output variable (O) with respect to the factors (F) (McCuen, 2003): ii s FF OO R / / 0   = (4-12) where 0 O is the value of O at some specified level of i F , i F are the factors that influence O , and s R is the relative sensitivity. Given the sensitivity of input F i with 34 respect to a variation in the output O of the system response, this equation determines the relative importance of the factor. The quantity s R from Equation 4-12, is an indication of the relative importance of the factor F i on predictions of O i . 4.2.5. Assessing Model Prediction Accuracy Model accuracy may be determined by examining the bias of the model, the standard error of the estimate, and the correlation coefficient. Bias is a statistical measure of the systematic variation of the errors of prediction. Positive bias exists if the model consistently overestimates the value being measured, and negative bias exists if the model consistently underestimates the value being measured. The general equation to calculate bias is:  == === n i n i ii i e n YY n ebias 11 1 )( 1 (4-13) A biased model exists if X is greater than zero and often indicates an incorrect model structure. The standard error of the estimate (S e ) is the square root of the sum of squares of the errors divided by the degrees of freedom. It is a measure of the nonsystematic error variation of the data. If the relationship between two variables is strong, then the standard error of the estimate will be smaller than the standard deviation of the criterion variable (S y ). The ratio of S e /S y is a measure of the relative improvement of the accuracy of predictions over predictions made with the mean of the variable. A low S e /S y is more acceptable than a value closer to one. The correlation coefficient (R) is a measure of the degree of linear relationship between two variables. This index measures the goodness of fit of the model. R 2 may 35 also be used as a measure of the accuracy of predictions made by the model; however, the standard error of the estimate is a better measure because it is valid for nonlinear models as well as linear models (McCuen, 1993). The following equation is used to estimate R: 5.0 2 1 1             = y e S S n n R  (4-14) where v is the degrees of freedom, and n is the sample size. 4.2.6. Sample Size Determination Three factors are used to determine sample size, two of which must be selected by the researcher: the tolerable error and the level of confidence. It is important to choose these values carefully because a large confidence interval will indicate an imprecise measure of the mean. In most situations, the level of confidence is set between 90-95% and the tolerable error is chosen arbitrarily by the researcher. The following equation was used to determine if the correct sample size had been chosen once the variance was known (Ott and Longnecker, 2001): 2 22 2/ )( E z n   = (4-15) where n = sample size, z Y/2 = z test statistic, Z 2 = population variance, and E = W/2 where W is the tolerable error. For the current study, the standard deviation (Z) was computed for each testing condition and an average of the computed standard deviations was taken. This value was 5.98. The tolerable error, which is representative of the width of the confidence interval of the mean, was arbitrarily chosen. If the width is too narrow and confidence level too 36 high, a large sample size will be necessary. Therefore, W = 6, and E = 3. A 95% confidence interval use used, which corresponds to a z value of 1.96. Therefore, the sample size necessary to be 95% confident that the population mean is contained in the interval, is: n = [(1.96) 2 *(5.98) 2 ]/3 2 = 15 Although 22 subjects are necessary for a confidence interval of 95% with a width of 5, due to time constraints, 10 subjects were chosen for participation in the study. Therefore, using a sample size of 10, the value of the z test statistic is: z Y/2 = (10 .5 *3 2 )/5.98 2 = 0.79 This z value corresponds to a confidence level of 78%. In order to improve this value to 95%, more subjects must be tested or the width of the interval (W) must be expanded to 7.5. 37 Chapter 5: Research Results and Discussion 5.1. Subject Demographics and Characteristics Ten healthy individuals participated in the research study: three female and seven male. A retrospective analysis of the sample size indicated that a sample size of 15 would have resulted in a 95% level of confidence. A sample size of 10 resulted in an 88% level of confidence. Sample size determination is discussed in section 4.2.6. The average age of the subjects was 26 + 7.3 years, the average height was 67.7 + 2.2 inches, and the average weight was 165.9 + 32 lbs (Table A1.1 in Appendix 1). The average VO 2 max of the subjects was 29.16 + 5.87 ml/kg/min (Table A2.1. in Appendix 1). All tests were randomized for each subject prior to commencement of the study. Three females and seven males participated in the study with ages ranging from 21 to 40. Of the ten subjects, four were familiar with respirator wear during manual labor. Three of these four subjects (001, 145, and 358) had performance times that followed the expected trend for each of the conditions. In other words, their shortest performance time occurred at the warmest condition, and the longest performance time occurred at the coolest condition. The other seven subjects had performance times that did not vary greatly as a function of the heat index. Although some of the subjects? performance times did not indicate that the warm and humid conditions affected them more than the more neutral conditions, all subjects were able to discriminate between the warmest (37?C, 70% RH) and the coolest (27?C, 50% RH) conditions. All subjects indicated that the warmest condition made breathing very difficult from the beginning and some felt as though they were suffocating, leading to termination. For the coolest condition, all 38 subjects indicated that breathing became difficult toward the end of exercise, but termination occurred due to fatigue, boredom, or leg and muscle pain. For the other two conditions (27?C, 70% RH and 32?C, 60% RH) subjects terminated for a variety of reasons including leg and muscle pain, fatigue, boredom, overall body discomfort due to heat, and difficulty breathing. 5.2. Sensitivity Analysis of Heat Index The heat index played a central part in this modeling effort. Therefore, it was important to understand how it functioned as a variable. For this reason, a sensitivity analysis of the heat index was undertaken prior to its use. The sensitivity of a model is useful for assessing the relative importance of the predictor variables and making error analyses. A sensitivity analysis was performed on the Heat Index equation developed by the National Weather Service (Equation 4-1) in order to determine how sensitive the equation was to changes in both temperature and humidity. The analyses were performed on temperatures ranging from 25?C to 40?C and humidities ranging from 40% RH to 80% RH. These bounds were selected as representative of normal test conditions. Both absolute and relative sensitivities were computed for temperature and humidity. The absolute sensitivity is the first derivative of the dependent variable with respect to the independent variable and is useful in error analyses. The relative sensitivity (Equation 4- 12) is the percentage change in the dependent variable for a 1% change in the independent variable; therefore, it is a useful indication of the relative importance of the independent variable. 39 As shown in Tables 5-1 and 5-2, a 1% change in the temperature at 40?C and 70% RH produced a 6.5% change in the actual heat index. A 1% change in relative humidity at 40?C and 70% RH caused a 2.3% change in the actual heat index. Therefore, at these values of T and RH, T was almost three times more influential than RH. This indicated that the commonly used heat index equation may not be the best option for analyses of data collected during testing. The coefficients of the heat index equation placed more emphasis on temperature than on the humidity. Table 5-1. Absolute and Relative Sensitivities for Temperature Temperature (?C) RH (%) 25 30 35 40 50 0.5824 1.4787 2.3719 3.2651 absolute 0.5735 1.6179 2.8668 4.3203 relative 60 0.8311 1.9061 2.9811 4.0561 absolute 0.8123 2.0808 3.5949 5.3546 relative 70 1.1861 2.4357 3.6853 4.9349 absolute 1.1594 2.6593 4.4446 6.5156 relative Table 5-2. Absolute and Relative Sensitivities for Relative Humidity Temperature (?C) RH (%) 25 30 35 40 50 0.0275 0.2829 0.705 1.294 absolute 0.0269 0.3095 0.8522 1.7122 relative 60 0.0085 0.359 0.8699 1.5411 absolute 0.0083 0.3919 1.049 2.0344 relative 70 -0.0105 0.4352 1.0347 1.7881 absolute -0.0102 0.4751 1.2479 2.3609 relative The absolute sensitivities in Tables 5-1 and 5-2 were also useful to show the effect of errors in measurements of T and RH. Mathematically, an error in T of ]T or in RH of ]RH can be used to compute the error in the heat index ]HI by: ]HI = ^HI/^T * ]T (5-1) ]HI = ^HI/^RH * ]RH (5-2) 40 Errors in values of a computed heat index were more sensitive to errors in recorded temperature than to errors in recorded humidity. For example, at 40?C and 70% RH, an error of 2?C led to a change of 9.8?C in the heat index. At 40?C and 70% RH, an error of 2% RH led to a 3.5?C change in the heat index. The analysis indicated that the Heat Index Equation was almost three times more sensitive to errors in temperature than to errors in relative humidity. 5.3. Performance Time ? Heat Index Model In order to predict performance time according to the heat index of environmental conditions, a model was fit to the data collected from each subject using the numerical least squares technique. All performance time data are in Table A1.3. in Appendix 1. Graphing the data indicated that the structure of the model was nonlinear and that performance time was negatively correlated with the heat index of the environmental condition. Two coefficients were used to define the model, which had the following form: HIC eCPT * 1 2  = (5-3) where PT=performance time (min), C 1 and C 2 are coefficients, and HI=heat index (?C). C 1 was the coefficient that represented the magnitude of the predicted values, while C 2 was the coefficient that described the rate of decline of the function as HI increased. Relatively high values of C 2 indicated a steep decline while relatively low values of C 2 indicated a gradual decline. Generally, the two coefficients were correlated such that an increase in C 1 occurred simultaneously with a decrease in C 2 . 41 Because the model was a nonlinear model, analytical methods for fitting C 1 and C 2 would not suffice; thus, numerical least squares was the appropriate technique. For this technique, the coefficients for the model were determined using an iterative, but systematic process, which resulted in minimization of the sum of the squares of the errors. The process involved numerically computing the derivatives of the objective function (Equation 5-3) and iteratively solving for the point where the derivatives were equal to zero (McCuen, 2003). Model results may be found in Table 5-3. Table 5-3. Model results for the performance time-heat index (PT-HI) model where PT=C 1 e -C2*HI . Subject S e S e /S y R 2 C 1 C 2 001 4.901 0.5634 0.7884 71.6 0.0358 145 0.306 0.0262 0.9995 140.7 0.05516 358 1.807 0.2627 0.954 58.95 0.03066 359 1.864 1.197 0.001 22.23 0.001219 379 1.001 1.193 0.001 13.75 0.001092 419 0.606 1.061 0.001 11.482 0.002157 420 1.213 1.174 0.001 14.442 0.00167 405 1.459 1.039 0.001 11.079 0.007195 401 0.89 0.57 0.7832 21.808 0.006405 343 0.555 0.15 0.9888 3.188 -0.04652 Model accuracy may be determined by examining the bias of the model, the standard error of the estimate, and the correlation coefficient. For the PT-HI model, all models resulted in a bias very close to zero, suggesting that the correct model structure had been chosen. The ratio of S e /S y is a measure of the improvement of the accuracy of predictions over predictions made with the mean of the variable. A low Se/Sy is more acceptable than a value closer to one. Subjects 001, 145, and 358 had low values of Se/Sy, indicating that the model accuracy for these three subjects was good. Because of sampling variation inherent to the measured data, five of the models were inaccurate as 42 evidenced from the high Se/Sy values and low R 2 values. These subjects had performance times that did not vary greatly with a change in the heat index of the environmental conditions resulting in flat curves and an R 2 value close to zero as shown in Figure 5-1. Calculations of performance time across a range of heat indices for each subject are in Appendix 5. 0 5 10 15 20 25 30 35 40 45 27 32 37 42 47 52 Heat Index (C) P T ( m i n ) PT (001) PT(145) PT(358) PT(359) PT(379) PT(419) PT(420) PT(405) PT(401) PT(343) Figure 5-1. Functions (n=29) for the PT-HI model (Equation 5-3) for all ten subjects. The square of the correlation coefficient (R 2 ) is a measure of the degree of relationship between performance time and the heat index. This index measures the proportion of variation explained by the model. For the subjects whose model accuracy was good, the goodness of fit of the model was very close to one. Subject 001 had an R 2 of 0.7884, subject 145 had an R 2 of 0.9995, and subject 358 had an R 2 of 0.954. Figure 43 5-2 shows the functions for each of the subjects whose model accuracy and goodness of fit were adequate. 0 5 10 15 20 25 30 35 40 45 50 27 32 37 42 47 52 Heat Index (C) P e r f o r m a n c e T i m e ( m i n ) PT(001) PT(145) PT(358) 001 145 358 Figure 5-2. Functions (n=29) for the PT-HI model (Equation 5-3) for subjects 001, 145, and 358 including original data points. It was evident from the PT vs. HI data that sampling variation can be very significant and adversely affect the modeling. The sampling variation was evident from comparing the performance times measured at 27?C and 28?C in Table A1.3 in Appendix 1. While these should not vary by much relative to the variation of the measurements made at 37?C and 55?C, for many subjects the PT differences for a 1?C change in HI were large. This would suggest that future studies might include replications to quantify the effects of sampling variation of each heat index. 44 0 5 10 15 20 25 30 35 40 45 50 27 32 37 42 47 52 Heat Index (C) P e r f o r m a n c e T i m e ( m i n ) PT(145) PT(419) 145 419 Figure 5-3. Comparison of Subject 145 (n=29) and Subject 419 (n=29). Model functions and original data points are shown. Subject 145 has a C 1 value of 140.7 and a C 2 value of 0.05516. Subject 419 has a C 1 value of 11.482 and a C 2 value of 0.002157. Figure 5-3 shows the relationship between a subject who was sensitive to the change in the heat index (Subject 145) and a subject who was insensitive to the change in the heat index (Subject 419). The function for subject 145 indicates a large difference in performance time for a heat index of 27?C versus a heat index of 55?C, whereas that for subject 419 shows almost no difference in performance times between these heat indices. Furthermore, the rate of decline for each function is indicative of the 25 fold difference in C 2 values. The shape of the PT-HI relationship reflected a number of characteristics about the subject on which the relationship was based. A steep decline was not necessarily indicative of good subject data, as the rate of decline can reflect the attitude and/or 45 physical capability of the subject; however, a flat curve generally indicated data insensitive to differences in the environmental condition. For example, subject 419 terminated each testing session except that at 55?C due to sore legs, not because the condition became difficult. Due to subject motivation, muscle soreness from other activities, and other external factors, only three subjects fit the model well. In order to obtain a better understanding for why this was the case, explanation of differences in C 1 was attempted using several subjective and objective parameters. Furthermore, once the differences had been accounted for, a new PT-HI model (Equation 5-9) was developed as a function of the new factors and combined all of the subjects. 5.4.Sample Graph Explanation 5.4.1. Rectal Temperature vs. Time Rectal temperature varied linearly with time and increased with a similar slope at each heat index value. Figure 5-4 shows how rectal temperature varied over time for all ten subjects. Each of the four heat index conditions showed little variation from one another, which indicated that rectal temperature increased independently of the environmental conditions, but nonetheless showed good correlation with time. Rectal temperature data for all heat indices are located in Tables A4.1. to A4.4. in Appendix 4. 46 R 2 = 0.9773 R 2 = 0.9948 R 2 = 0.9949 R 2 = 0.9884 37.2 37.3 37.4 37.5 37.6 37.7 37.8 37.9 38 0246810 Time (min) R e c t a l T e m p ( C ) 27, n=21 28, n=16 37, n=14 55, n=13 Figure 5-4. Average rectal temperature vs. time for all four heat index values. R 2 =0.9884 for HI=27?C, R 2 =0.9773 for HI=28?C, R 2 =0.9949 for HI=37?C, and R 2 =0.9948 for HI=55?C 5.4.2. User Acceptability vs. Time User acceptability of the respirator was measured using the BACS scale (0-10) with 0 indicating very, very uncomfortable conditions and 10 indicating very, very comfortable conditions. All comfort scales are located in Appendix 9. The scale was converted to a percentage to measure the acceptability of each of the heat index conditions using the following method: (BACS score/10)*100 (5-4) During each testing session, each subject expressed his or her level of discomfort every 2 minutes using the BACS scale. This data is located in Tables A3.1. to A3.4. in 47 Appendix 3. Averages were taken for each heat index across all ten subjects. Table 5-4 shows these values for each of the four heat indices. Table 5-4. Average of user acceptability (%) for each of the subjects at the four heat index conditions. HI=27 HI=28 HI=37 HI=55 Time (min) Acceptability (%) Acceptability (%) Acceptability (%) Acceptability (%) 0 72 75 75 66 2 55 61 52 43 4 46 48 41 29 6 41 39 36 26 8 32 32 24 25 10 24 29 21 12 19 The subject acceptability data of Table 5-4 were plotted in Figure 5-5 to show how acceptability declined over time. However, the effect of the heat index was very minimal, since the trend at each heat index was nearly identical. At any time, differences in acceptability were evident, but the trend was not consistent. For example, at a time of 0, the order was 28?C and 37?C (tie), then 27?C, and then 55?C. At a time of 8 minutes, the order was 27?C and 28?C (tie), then 55?C, then 37?C. 48 0 10 20 30 40 50 60 70 80 90 100 0 2 4 6 8 10 12 14 Time (min) A c c e p t a b i l i t y ( % ) 27, n=7 28, n=6 37, n=5 55, n=6 Figure 5-5. Average user acceptability (%) vs. time (min) for each of the four heat index conditions. A model was developed to represent the relationship between acceptability and time. A comfort level of 40% corresponded to a BACS score of 4, ?fairly uncomfortable.? Any scores below this level indicated that the subject felt that the respirator was uncomfortable. An equation was derived to predict the amount of time in minutes it took for a person to deem the respirator unacceptable based on the heat index of the environmental condition. Graphical analyses such as in Figure 5-5 indicated that the time (T, minutes) to reach a condition of unacceptability varied as an exponential decay function with the acceptability index (A,%), and that the rate coefficient of the exponential function varied approximately linearly with the heat index (HI). These trends suggested the following model: 49 ))**(( 321 AHI eT  ++ = (5-5) where T=time (minutes), _ 1 , _ 2 , and _ 3 are model coefficients, HI=heat index (?C), and A=acceptability (%). Raw data (Table A3.5.) and functions (Figure A3.1.) showing the relationship between acceptability level and time for the four heat index conditions are found in Appendix 3. The empirical coefficients _ 1 , _ 2 , and _ 3 were fitted using numerical least squares analyses of measured values of T, A, and HI. The data of Table 5-4 were used to fit values of _ 1 , _ 2 , and _ 3. An initial analysis showed that the data for a heat index of 27?C did not follow the trend expected; specifically, some of the acceptability values for 27?C are lower than the values for 28?C at the same time. The model, including constants took the form: ))**00056.002784.0(39.3( AH I eT + = (5-6) where T is the predicted value of the time to unacceptability (min), and A is the level of acceptability (%). Equation 5-6 over-predicted measured times by 0.13 minutes. The standard error estimate of Equation 5-6 was 0.94 minutes (standard error ratio = 26%). This suggested that the model provided excellent accuracy. The correlation coefficient was 0.97 or 94% explained variance. These goodness of fit statistics indicated that the model provided accurate estimates of the time to unacceptability. Figure 5-6 shows the relationship of Equation 5-6 between the time to reach an unacceptable level according to the heat index of the environmental condition for three levels of percent acceptability. 50 0 1 2 3 4 5 6 7 8 9 10 27 32 37 42 47 52 Heat Index (C) T i m e ( m i n ) 30% 40% 50% Figure 5-6. Model functions (n=29) for predicting time to reach unacceptable levels of comfort of the respirator according to the heat index of the environmental condition for acceptabilities of 30, 40, and 50%. The model equations indicated that the respirator became uncomfortable at a level of 40% at 2.85 minutes for the heat index of 55?C, at 4.26 minutes for the heat index of 37?C, at 5.22 minutes for the heat index of 28?C, and at 5.34 minutes for the heat index of 27?C. Overall, the time to reach an unacceptable level of respirator comfort decreased with an increase in the heat index of the environmental conditions, indicating that there was an effect of the condition on respirator comfort. The time at which the respirator becomes uncomfortable will provide useful information for those who set policies on the length of time at which the performance of respirator users might decline substantially. The time to reach an unacceptable level of respirator comfort was not considered to be representative of termination time. The model may serve as a useful tool for both respirator manufacturers and employers to determine how long it takes for the respirator 51 user to become uncomfortable in the environmental conditions. This time may be indicative of deteriorating physiological function at the worksite and decreased work efficiency. While some individuals may push through the discomfort and continue to perform, others may terminate or remove the respirator at this time. 5.4.3. User Acceptability vs. Mean Facial Skin Temperature User acceptability was measured in the manner described in section 5.4.2. In order to compare the results obtained from the current study with those in the literature, user acceptability was graphed against mean facial skin temperature for all ten subjects at each of the four heat index values. Figure 5-7 shows this relationship. Data for mean facial skin temperature is located in Tables A2.1. to A2.4. in Appendix 2. An overall relationship between user acceptability and mean facial skin temperature was evident. It was expected that at the higher heat index values, acceptability would decrease more quickly as facial skin temperature increased, but this was not observed; however, as mean facial skin temperature increased for all four conditions, acceptability decreased. 52 20 30 40 50 60 70 80 33.6 33.8 34 34.2 34.4 34.6 34.8 35 35.2 Mean Skin Temp (C) A c c e p t a b i l i t y ( % ) 27, n=6 28, n=5 37,n=5 55,n=4 Figure 5-7. Average user acceptability (%) versus average mean facial skin temperature (?C) for all four heat index values. In previous studies (Gwosdow et al., 1989; Dubois et al., 1990; Fox and Dubois, 1993), exercising subjects with lip skin temperatures of 31?C or less indicated that the condition was almost 100% acceptable and any temperature over this threshold became increasingly unacceptable. In the current study, a mean facial skin temperature (a weighted mean of lip, cheek, and forehead temperatures) was used instead of the lip temperature since Gwosdow et al. (1989) indicated that the cheeks and forehead are the regions most sensitive to warm stimuli. Dubois et al. (1990) fit a linear regression through the data points representing the relationship between acceptability and mean facial skin temperature. A similar approach was taken in order to compare results obtained from the current research study to those 53 obtained by Dubois et al. (1990). Dubois et al. (1990) obtained the following equation for unacceptability of mask conditions: A=-16.6347+0.527*F (5-7) where A=unacceptability (%), and F=mean facial skin temperature (?C) (left nasolabial fold). Both nonlinear and linear regression equations were fit to the data collected in the current study; however, the linear model produced the best results. The model took the following form to describe acceptability of mask conditions: A=1362.5-0.2*HI-38.40*F (5-8) where A = acceptability (%), HI = heat index (?C), and F = mean facial skin temperature (?C). The standard error estimate of Equation 5-8 was 8.74 (standard error ratio 52%). This suggested that the model provided good accuracy. The correlation coefficient was 0.87 or 74% explained variance. These goodness of fit statistics indicated that the model provided accurate estimates of acceptability as a function of the heat index and mean facial skin temperature. Dubois et al. (1990) concluded that acceptability began to decrease and the respirator mask became uncomfortable above mean facial skin temperatures of 34.5?C. In the current study, the threshold of acceptability was defined as a value of 40%, which corresponded to a BACS score of 4 (?fairly uncomfortable?). Table 5-5 shows that for all heat index values, the respirator became uncomfortable above facial skin temperatures of 34.5?C. Figure 5-8 illustrates this result. Beyond this temperature, acceptability fell below 40%. This result confirmed those obtained by Dubois et al. (1990). 54 Table 5-5. Acceptability (%) values computed from Equation 5-8 for 27?C, 28?C, 37?C, and 55?C for a range of mean facial skin temperatures. Skin Temp (?C) HI=27?C HI=28?C HI=37?C HI=55?C 32.9 98.6 98.58 98.4 98.04 33 94.76 94.74 94.56 94.2 33.1 90.92 90.9 90.72 90.36 33.2 87.08 87.06 86.88 86.52 33.3 83.24 83.22 83.04 82.68 33.4 79.4 79.38 79.2 78.84 33.5 75.56 75.54 75.36 75 33.6 71.72 71.7 71.52 71.16 33.7 67.88 67.86 67.68 67.32 33.8 64.04 64.02 63.84 63.48 33.9 60.2 60.18 60 59.64 34 56.36 56.34 56.16 55.8 34.1 52.52 52.5 52.32 51.96 34.2 48.68 48.66 48.48 48.12 34.3 44.84 44.82 44.64 44.28 34.4 41 40.98 40.8 40.44 34.5 37.16 37.14 36.96 36.6 34.6 33.32 33.3 33.12 32.76 34.7 29.48 29.46 29.28 28.92 34.8 25.64 25.62 25.44 25.08 34.9 21.8 21.78 21.6 21.24 35 17.96 17.94 17.76 17.4 35.1 14.12 14.1 13.92 13.56 35.2 10.28 10.26 10.08 9.72 35.3 6.44 6.42 6.24 5.88 35.4 2.6 2.58 2.4 2.04 55 Figure 5-8. Plot of average acceptability vs. average mean facial skin temperature using data from Table 5-5 for the four heat index conditions (n=26). The results indicated that respirator comfort decreased above a mean facial skin temperature of 34.5?C during exercise conditions, which confirmed the results of Dubois et al. (1990). This information will be useful for employers who may choose to monitor facial skin temperature as an indication of respirator comfort and ultimately, work efficiency. 5.4.4. Mean Facial Skin Temperature versus Time Figure 5-9 shows the measured data of the mean facial skin temperature as a function of time. The data are found in Tables A2.1. to A2.4. in Appendix 2. The curves show an initial plateau or decline in facial skin temperature followed by a linearly increasing trend. The plateau or decline did not represent significant variation, but the increasing trend was meaningful. A substantial difference between each of the four 56 conditions was not evident; however, the general relationship indicated an increase in facial skin temperature over time. At 37?C, the slope in the linear portion of the data was much higher than that of 55?C. This could be a result of lower performance times during testing at 55?C, during which the mean facial skin temperature never reached a peak. 33.50 33.70 33.90 34.10 34.30 34.50 34.70 34.90 35.10 024681012 Time (min) M e a n F a c i a l S k i n T e m p ( C ) 27, n=22 28, n=16 37, n=17 55, n=13 Figure 5-9. Average mean facial skin temperature (?C) vs. time (min) for all four heat index conditions. 5.4.5. Rectal Temperature vs. Mean Facial Skin Temperature Figure 5-10 shows the fitted relationships between the mean facial skin temperature and rectal temperature. The graph was of value in characterizing the rate of increase of rectal temperature in response to the environmental stimulus. At 27?C, the rectal temperature correlated to mean facial skin temperature (p<0.05) with an R 2 value 57 of 0.9775. At 28?C, rectal temperature correlated to mean facial skin temperature (p<0.05) with an R 2 value of 0.9139. At 37?C, rectal temperature correlated to mean facial skin temperature (p<0.05) with an R 2 value of 0.9472. At 55?C, rectal temperature correlated to mean facial skin temperature (p<0.05) with an R 2 value of 0.9395. As the heat index increased, the temperature range decreased. This was mainly due to the fact that at the higher heat index values where performance time was shorter, less data were collected. At a heat index of 55?C, the slope of the trend was .67. At heat index values of 37?C and 28?C, the slope was .31. At a heat index of 27?C, the slope was .47. This indicated that mean facial skin temperature and rectal temperature increased independently of the environmental condition. R 2 = 0.9395 R 2 = 0.9472 R 2 = 0.9775 R 2 = 0.9139 37.2 37.3 37.4 37.5 37.6 37.7 37.8 37.9 38 33.6 33.8 34 34.2 34.4 34.6 34.8 35 Mean facial skin temperature (C) R e c t a l t e m p e r a t u r e ( C ) 27, n=24 28, n=18 37, n=14 55, n=13 Figure 5-10. Average rectal temperature vs. average mean facial skin temperature for all four heat index values as well original data points. 58 5.5. Analysis of Factors Affecting C 1 Parameter C 1 from Equation 5-3 was found to vary among data representing different subjects and test conditions. Several surveys (section 2.8) were administered to the subjects and analysis of these surveys resulted in numerical values that varied according to the responses of the subject. Responses to the surveys are in Tables A6.1. to A6.5 in Appendix 6. In order to determine variants on which C 1 depended, a series of graphical procedures was undertaken. These plots (Figures A7.1. to A7.9) are in Appendix 7. A relationship was not evident between C 1 and all survey parameters; C 1 did have a relationship with minute volume, the sensing-intuition (SN) personality characteristic, and respirator familiarity. The relationship between C 1 and minute volume was the first of these. 5.5.1. C 1 versus Minute Volume Minute volume for each of the subjects is located in Table A1.2. in Appendix 1. This value represents the minute volume recorded for each subject at the work rate corresponding to 65-70% of their VO 2 max as determined during the VO 2 max test. Figure 5-11 shows the relationship between the C 1 and minute volume (V e ). Minute volume was essentially a measure of a person?s capacity to breathe air, and it was measured in liters of air inspired per minute. Larger individuals and those individuals who were very physically active typically had higher minute volumes than those who were smaller and less physically active. Figure 5-11 indicates that females typically had lower minute volumes than the men at their particular work rate as a percentage of 59 VO 2 max during the study, and that a slight relationship existed between C 1 and V e for the men. The data shown in Figure 5-11 suggested an increase in C 1 with increasing V e. 0 20 40 60 80 100 120 140 160 0 1020304050607080 Ve (L/min) C 1 Female Male Figure 5-11. C 1 (from Equation 5-3) vs. V e for female and male subjects (n=10). 5.5.2. C 1 versus SN Figure 5-12 shows the relationship between C 1 and the Sensing-Intuition (SN) parameter from the MBTI assessment. The results indicated that those with higher C 1 values also exhibited a lower tendency to have sensing characteristics, and instead had higher intuitive characteristics. According to Figure 5-12, having more intuitive characteristics should increase the propensity of that individual to fit the initial PT-HI model (Equation 5-3); however, the opposite result was expected. When modeled collectively (Equation 5-9), C 1 and SN 60 showed a positive relationship instead of a negative one. 0 20 40 60 80 100 120 140 160 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 SN C 1 Figure 5-12. The relationship between the model coefficient C 1 (from Equation 5-3) and SN for all subjects (n=10). The negative relationship of this bivariate plot was misleading because other more dominant variables also influenced the value of C 1 . 5.5.3. C 1 vs. Respirator Familiarity Respirator familiarity was quantified as the number of times an individual had worn the respirator while performing manual labor prior to the research study. Figure 5- 13 shows the relationship between C 1 and respirator familiarity. As an individual became more familiar with the respirator, they were also more inclined to have a better fit with 61 the PT-HI model, which was indicated by a higher C 1 value. 0 20 40 60 80 100 120 140 160 0 10 20 30 40 50 60 70 80 90 # of times Respirator worn C 1 Figure 5-13. The relationship between the model coefficient C 1 (from Equation 5- 3) and Respirator Familiarity for all subjects (n=10). 5.6. Collective PT-HI model In reviewing the fitted coefficients of the original model (Equation 5-3) and knowing the subjects, it was evident that variations of the coefficients were related to characteristics of the subject. In order to assess this variation, a number of parameters were analyzed, three of which showed strong correlations with the original model coefficients. These parameters included psychological type, respirator familiarity, and minute volume. The performance time-heat index model was modified to include the sensing- intuition (SN) psychological parameter, the number of times the respirator was worn 62 prior to the study (RF), and the minute volume (V e ). This collective model accounted for the variations in performance times among each of the subjects and provided accurate reproductions of the performance time. In order to predict performance time according to the heat index of environmental conditions, an empirical model was fit to the data collected from each testing session using the numerical least squares technique. Graphing the data indicated that the structure of the model was nonlinear and that performance time was negatively correlated with the heat index of the environmental condition. A significant amount of variation existed between the subjects as evidenced from the differences between C 1 from Equation 5-3. Thus, in order to obtain an accurate model for performance time, several parameters were evaluated using surveys to determine how each affected the performance time. The parameters chosen that affected C 1 were minute volume (V e ), the Sensing-Intuition characteristic (SN), the number of times the respirator was worn (RF), and the heat index (HI). The general form of the model is as follows, and model results are in Table 5-6: HIRFSN e eeeVPT *** 1 5432  = (5-9) where PT=performance time (min), Y 1 , Y 2 , Y 3 , Y 4 , and Y 5 are model coefficients, V e =minute volume (L/min), SN=sensing-intuition parameter, RF= number of times the respirator was worn, and HI=heat index (?C) Table 5-6. Performance time model results from Equation 5-9. n e/y S e S e /S y R Y 1 Y 2 Y 3 Y 4 Y 5 40 -0.004 4.15 0.6141 0.8161 14.86 -0.1243 1.293 0.01624 -0.0198 (n= sample size, e/y = bias, S e = standard error of the estimate, S e /S y = prediction accuracy, R = correlation coefficient, Y 1 , Y 2 , Y 3 , Y 4 , and Y 5 are model coefficients) In reviewing the residuals of the model, the prediction accuracy and goodness of fit statistics were improved over those shown in Table 5-3 from Equation 5-3. While the 63 goodness of fit statistics were not as good as they could be, they indicated that a collective model to include all subjects as well as other significant parameters was a good representation of the PT-HI process. Some of the subjects had performance times that did not vary greatly with a change in the heat index of the environmental conditions. Initially, the majority of subjects had poor goodness of fit statistics, with Se/Sy values above 1.0 and correlation values close to zero. However, when the subjects were modeled collectively as a function of V e , SN, RF, and HI, the explained variation rose substantially. The individual effects of each variable are shown in Table 5-7. Performance time was calculated over a range of each variable while holding the other variables at mean values. Table 5-7. Performance time (min) predictions from Equation 5-9 for values of V e (L/min), SN (ratio), RF (number of times respirator worn), and HI (?C) Ve PT (min) SN PT (min) RF PT (min) HI PT (min) 20 16.08 0.2 8.47 0 8.73 25 19.51 30 15.29 0.3 9.64 10 10.27 30 17.67 40 14.75 0.4 10.97 20 12.08 35 16.01 50 14.35 0.5 12.49 30 14.21 40 14.50 60 14.03 0.6 14.21 40 16.72 45 13.13 70 13.76 0.7 16.17 50 19.67 50 11.89 80 13.53 0.8 18.41 60 23.13 55 10.77 0.9 20.95 70 27.21 80 32.01 To show the effect of each variable, the model (Equation 5-9) was used to develop plots of each variable. The values of the four variables were varied over the approximate range of the measured data. The range of the computed values of performance time shown in the graphs suggested the importance of that variable. Figures 5-14 through 5-17 include estimations of performance time for each parameter listed in Table 5-7. 64 13 14 14 15 15 16 16 17 20 30 40 50 60 70 80 Ve (L/min) P e r f o r m a n c e T i m e ( m i n ) Figure 5-14. Performance time (minutes) vs. V e (L/min) computed from Equation 5-9. Performance time decreased with an increase in an individual?s minute volume (V e ) . This may be explained by differences in size, sex, and level of physical activity. Minute volume is the amount of air an individual inhales per minute. Typically, a person who is of smaller stature has a lower minute volume since their lung capacity and need for air are smaller. Furthermore, larger individuals may have different heat transfer characteristics from their bodies than someone who is of smaller stature. Thus, someone with a smaller minute volume probably is not going to feel the effects of an extreme environmental condition as intensely as a person who inhales a great deal of air per breath. 65 0 5 10 15 20 25 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 SN (ratio) P e r f o r m a n c e T i m e ( m i n ) Figure 5-15. Performance time (minutes) vs. the sensing-intuition (SN) parameter computed from Equation 5-9. When modeled collectively in Equation 5-9, SN showed a positive relationship with performance time. Performance time increased with an individual?s propensity to a higher SN ratio as determined from the MBTI assessment. A person with a high ratio of SN possessed more sensing (S) characteristics as compared to intuition (N) characteristics. This individual is likely to deal in facts and has an attitude that places emphasis on the present, rather than the future. This individual tends to focus on activities at hand. Because the N-type person is generally a daydreamer, they lack focus on activities in the present. Therefore, performance time was expected to increase with an increase in values of SN, and the model coefficient related to SN showed the expected trend. This relationship seems rational, since a person who thinks about current activities 66 is more likely to stay focused on their performance and tends to have a longer performance time than a person who is already thinking about their next task. 0 5 10 15 20 25 30 35 0 10 20 30 40 50 60 70 80 # of times respirator worn P e r f o r m a n c e T i m e ( m i n ) Figure 5-16. Performance time (min) vs. the number of times of the respirator was worn during manual labor prior to the current study computed from Equation 5-9. Each of the subjects was asked to disclose the number of times he or she had previously worn the respirator while performing manual labor prior to participation in the current study. This number was used to quantify an individual?s level of familiarity with the respirator. According to the results from Figure 5-16, an increase in respirator familiarity increased a person?s performance time at any given environmental condition. An individual?s confidence in a test condition directly influences their performance. An individual?s familiarity with the respirator mask imparts confidence in their ability to perform while wearing a mask. Respirator masks are uncomfortable due 67 to the design components necessary for adequate protection; a person who is familiar with this discomfort likely performs better than a person who is not familiar with the respirator. The novice can be overwhelmed by the discomfort and has uncertain expectations. This lack of confidence likely leads to a lower level of performance. Someone who has worn the respirator repeatedly while performing physical labor will likely push through the discomfort and only terminate because of the limitations the environmental condition placed upon them. Performance time decreased with an increase in the heat index of an environmental condition. The decay coefficient of the heat index component of the model exhibited the expected direction. 0 5 10 15 20 25 27 32 37 42 47 52 Heat Index (C) P e r f o r m a n c e T i m e ( m i n ) Figure 5-17. Performance time (minutes) as a function of the heat index (?C) of the environmental condition computed from Equation 5-9. Figure 5-17 shows the range of PT for a range of heat indices while holding the other variables at their means. This result was expected since exercising in a very warm, 68 humid environment compared to one that was cooler and less humid stressed the subject on a number of levels, forcing them to terminate sooner. Inside the respirator mask, evaporation was inhibited, leading to a build-up of saturated air from the respiratory system. This increased facial skin temperature, making the subject feel unbearably warm. Furthermore, breathing humid air in comparison to air that was less humid put a strain on the respiratory system, perhaps forcing labored breathing and a feeling of suffocation. All subjects felt as though they were hyperventilating and were not getting enough air during the warmest condition, citing difficult breathing as the reason for termination. Figure 5-18. Predicted performance time (n=4) vs. observed performance time (n=4) at the four heat index conditions. Each observed performance time is an average of the performance times for ten subjects. 69 Figure 5-18 shows the trend for predicted performance times from the model (Equation 5-9) versus average values of the observed performance times. The R 2 value was 1.0 indicating a good linear relationship. The slope was 0.99, indicating that the model predicts performance time well. Table 5-8 displays the observed and predicted values for performance time. Table 5-8. Predicted performance time values (min) and observed average performance time values (min) for ten subjects. HI (?C) Predicted PT (min) Observed PT (min) 27 18.1 18.1 28 18.5 18.5 37 15.3 15.3 55 12.3 12.3 The model (Equation 5-9) will be useful for manufacturers because it can help them to design respirators appropriate for the locations and environments under which they will be used. This may include attaching motorized blowers with more efficient filters to lower the humidity inspired by the user or designing masks with enhanced evaporative cooling. Such a model would be useful for employers to determine the factors that contribute to the detriment of performance time as the inspired air conditions become warmer and more humid. Employers may use the model for pre-screening purposes and for determining how long they can expect their employees to perform efficient work. Knowledge of an individual?s V e , familiarity with the respirator, and/or SN-type personality, can allow employers to gauge the performance time of workers under a variety of environmental conditions. The PT-HI model (Equation 5-9) predicts performance time across a range of heat indices; however, the performance time values are all less than 20 minutes. While an 70 individual may not stop working after this time, they may remove the respirator, exposing them to toxic substances. Employers must be aware that this is occurring, and thus they may choose to allocate their human resources more effectively. Furthermore, the model would be useful in respirator training programs to help illustrate expected performance. Ultimately, the model can result in increased comfort of respirators, better work efficiency, and less injury and heat related illness of respirator users. The PT-HI model (Equation 5-9) should be used with some caution. The R value was 0.82, meaning that performance time can not always be accurately predicted from V e , RF, SN, or HI. An R value closer to 1.0 would be indicative of a better model; including more parameters to better explain the variation among subjects would likely achieve this. However, the collective model (Equation 5-9) had better prediction accuracy than the original model that included only the heat index as evidenced in Table 5-3. This is an indication that data relative to individual subject characteristics must be collected in order to explain the performance of individuals performing work in warm, humid environments. 5.6.1. Sensitivity Analysis of the PT-HI model (Equation 5-9) The performance time model of Equation 5-9 included four variables, each representing a different characteristic of the system. The four predictor variables represented the environmental conditions (HI) of the test, the experience (RF) of the subject, the physiological stature (V e ) of the subject, and the psychological nature (SN) of the subject. To assess the importance of these four factors, a relative sensitivity analysis 71 of the PT-HI model (Equation 5-9) was made. The four relative sensitivity functions are as follows: ^PT/^V e * V e /PT = Y 2 (5-10) ^PT/^SN * SN/PT = Y 3 SN (5-11) ^PT/^R * RF/PT = Y 4 RF (5-12) ^PT/^HI * HI/PT = Y 5 HI (5-13) where the coefficients Y 2 , Y 3 , Y 4 , and Y 5 are the values given in Equation 5-9. While the relative sensitivity functions can be evaluated at any level of a variable, the mean values are most commonly used. Based on the data for the ten subjects of this research, the results of a sensitivity analysis are summarized in Table 5-8. The results showed that the environmental (HI) and psychological (SN) factors were the most important, with relative sensitivities of more than 80%. Experience with the respirator (RF) was less important, but a 1% increase in respirator use will lead to about a 0.5% increase in PT. The physiological factor (V e ) was the least important, but it was still important, with a relative sensitivity of about 30%. The relative sensitivities appeared to give rational indications of the relative importance of the four predictors and general intent of the effects. Table 5-9. Relative sensitivity analysis of the PT-HI model (Equation 5-9). Variable Mean PT Model Coefficient Model form Relative Sensitivity Function Relative Sensitivity SN 0.57 1.561 exponential Y 3SN 0.89 HI 36.75 -0.0231 exponential Y 5HI -0.849 R 29.65 -0.0183 exponential Y 4RF 0.542 V e 54.08 -0.315 power Y 2 -0.3165 72 Chapter 6: Conclusions Performance time correlated strongly with V e , SN, RF, and HI. Physiological variables such as rectal temperature and facial skin temperature did not vary significantly with the change in the environmental conditions. A sensitivity analysis of the PT-HI model indicated that the most important predictors of performance were the heat index and personality type, followed by respirator familiarity, then minute volume. Individual subject characteristics were more important than physiological variables as predictors of performance times. The respirator became uncomfortable above a mean facial skin temperature of 34.5?C Times to reach various levels of acceptability at the environmental conditions were determined 73 Chapter 7: Recommendations for Further Research 7.1 Equipment During the course of the research several problems with the environmental chambers arose. Because they were several years old, the compressors and chillers failed repeatedly and this forced testing to be delayed for several months. A solution to this problem would be to design a suitable environmental chamber that could supply warm humid air to the respirator mask and obtain equipment that would perform properly. The humidity chamber could be constructed similar to the device (Figure 2-1) built by Gwosdow et al. (1989), with some modifications made to enhance the ability of the air to become saturated more quickly. Furthermore, the device should be capable of supplying the subject with the appropriate flow rates conducive to moderate intensity exercise. According to Wilmore (2004), the maximum peak flow rate of large men is on the order of 100 L/min, although the average flow rate is around 24 L/min. A Plexiglas chamber would be filled with the volume of water needed to supply the specified humidity and air temperature within the chamber. A heating component capable of controlling the temperature of the system would consist of several coils. Insulated tubing would connect the outlet of the humidity chamber with the inlet of the respirator mask in order to minimize heat losses. A fan system should be used to push air into the chamber, which would pass through a series of grids on the lid of the chamber to allow the air to pass through slowly, and thus, allow for maximum saturation to occur. 74 The outlet of the chamber should be connected directly to an insulated hose, which in turn, connects to the inspiratory valve of the respirator. A suitable system designed to deliver warm humid air to the respirator mask would be another suitable method for subject testing considering the equipment problems experienced with the commercial environmental chambers used for this research. The temperature and humidity sensors used to monitor expired air inside the respirator masks were not sufficiently quick to display instantaneously the temperature and humidity. However, the sensor did reflect a relative change in temperature and humidity over the course of the testing session. A sensor capable of displaying breath by breath changes in temperature and humidity would be preferred in order to separate and calculate heat losses from the face and respiratory system. 7.2 Subject Effort Performance time was directly related to subject effort, which may be a function of several variables. Since performance time was the independent variable in the model, it was necessary to attempt to explain the variables that may influence subject effort. The inability to control the subject effort can introduce significant errors into the data collected. Subject effort variables include, but are not limited to, subject motivation, familiarity with the respirator, level of physical fitness, emotional state prior to testing, and personality type. If each of these variables could be controlled, a more accurate model could be developed. Subject motivation may arguably be the strongest predictor for how well a person is able to perform work; however, it is also the hardest to quantitatively measure. 75 Existing indices are purely subjective and may be a function of the person?s mood, overall attitude, physical health, stress level, and diet. A questionnaire could be administered to measure a person?s motivation based on the aforementioned factors, but it is likely to change on a daily basis and thus must be administered before each testing session. A quantitative score should be given to each subject for each testing session completed in order to measure their personal motivation for performing the work. It may also be necessary to administer a second test immediately after testing to determine if the subject?s motivation has waned, as it may for an intuitive (N) personality type. Four out of the 10 subjects tested for this study were unfamiliar with wearing the respirator. In other words, they had worn a respirator less than 10 times while performing physical labor. Because a respirator is uncomfortable to begin with, a person who is not familiar with this feeling may be more inclined to terminate because of this discomfort before the effects of the environmental condition can be felt. Furthermore, one of the subjects tested was very familiar with the respirator. He was a member of the armed forces for 7 years, and frequently donned the respirator for several hours at a time in extreme environmental conditions. During this time, regardless of the discomfort, he was unable to remove the respirator and was forced to endure the condition. Therefore, during testing he pushed through each of the conditions with relative ease and did not cite the conditions as his reason for termination. The remaining five subjects had worn the respirator several times while exercising in previous studies in the lab and were, therefore, moderately familiar with the discomfort of wearing a respirator. Attempting to quantify the familiarity with wearing a 76 respirator may help to explain the performance time of each individual. Therefore, research is needed to more accurately quantify respirator mask familiarity as a predictor. A third factor that may be related to subject effort is the level of physical fitness. The body temperature of a person who is out of shape may increase more rapidly. The person may become dehydrated more quickly and may be inclined to terminate sooner than someone who is more physically fit. A quantitative measure of a person?s physical fitness level would help to explain differences in performance time under each of the testing conditions. This measure may include the duration and frequency of exercise and type of exercise performed. 7.3. Research Methods It is imperative to obtain an accurate representation of the population when performing a research study. The number of subjects affects the accuracy of conclusions made from the measured data, with accuracy increasing with the sample size. However, it is also important to ensure that subjects show variability in the important factors. Both the sample size and the variability inherent to the data collected determine knowledge gained from the data analysis. While only ten subjects were tested in this research, the subjects varied in physical fitness, personality type, aerobic capacity, and mask familiarity. This enabled the research to identify important relationships. However, more knowledge about the population could be gained by testing more subjects. These subjects should include equal numbers of men and women, they should represent all levels of physical fitness, and represent both subjects who are familiar with wearing a respirator and those who are unfamiliar with wearing a respirator. By including subjects with diverse characteristics the analyses are more likely to detect the true effects of the 77 inputs. Furthermore, in order to validate the model, more tests on the ten subjects who participated in the study are needed. Since each subject has a different VO 2 max, V e , and tidal volume (V t ), it is possible that the amount of oxygen utilized and amount of air intake per minute could help to explain why some subjects terminate sooner than others at each of the testing conditions. For example, some of the larger subjects seemed to be affected by the conditions more so than some of the smaller subjects. This could be a direct function of how much air is being utilized per minute. The larger subjects were perhaps feeling the warm humid conditions sooner and more intensely than the smaller subjects because they were breathing in a larger volume of air per breath. If continuously expired air data were monitored during each testing session, this could provide the researcher with more information regarding the relationship between such parameters as minute volume (V e ) and tidal volume (V t ) to performance time under the environmental conditions. In order to gain a better understanding of each subject?s attitude prior to each testing session, a state anxiety test could be administered. This test would give the researcher information on how the subject is feeling that day, and a quantitative score is reported. A low score indicates low anxiety and a high score indicates high anxiety. Since a person feels differently day to day, this score could provide some insight on why a subject performs particularly well on one day, and poorly on the next day, especially when the difference is not related to the change in the environmental condition. Such inconsistency in performance introduces error into the measured data. Such error could mask effects or suggest significant effects that are not accurate. 78 The performance time ? heat index model was developed from exercise in neutral-warm conditions. In order to develop a more accurate model capable of explaining how performance time could be predicted for exercise in cooler environments, three to four cool, dry conditions should be added to the protocol, and the analyses repeated. The model developed indicates a negative correlation between performance time and the heat index of the condition; however, a more accurate indication of the underlying function could be obtained with a wider variation in the data. A parabolic function may be observed if the cooler conditions were included in the protocol. 79 Appendix 1 (Subject Excel Data) Table A1.1 Subject Demographics Subject Age Height (in) Weight (lb) Sex 001 43 68 205 M 145 35 70 209 M 343 21 70 190 M 358 24 66 142 F 359 26 70 180 M 379 23 65 135 F 401 25 64 110 F 405 21 69 175 M 419 21 68 148 M 420 21 67 165 M Average 26 67.7 165.9 St. Dev 7.3 2.2 32 Table A1.2. VO 2 max data Subject VO 2 (ml/m/kg) Ve (L/min) Vt (L) 145 28.7 67.61 1.64 420 34.2 51.86 1.9 001 25.53 64.79 1.31 419 29.27 51.85 1.28 359 29.26 53.09 1.64 358 22.9 34.93 1.04 379 18.09 27.8 1.02 343 36.42 74.85 1.86 401 36.57 47.12 1.24 405 30.65 61.04 1.27 Average 29.2 53.5 1.4 St. Dev 5.8 14.5 0.3 Table A1.3. Performance Time data Condition (?C) Performance Time (min) Subject 001 27 24.12 28 31.22 37 15.75 55 11.75 Subject 145 27 31.75 28 29.88 37 18.58 55 6.5 80 Condition (?C) Performance Time (min) Subject 358 27 25.25 28 26.42 37 17.17 55 11.92 Subject 359 27 19.7 28 23.40 37 21.20 55 20.75 Subject 379 27 12.5 28 13.53 37 14.25 55 12.56 Subject 419 27 11.43 28 10.50 37 10.11 55 10.40 Subject 420 27 15 28 12.56 37 13.75 55 13.22 Subject 405 27 10.65 28 7.63 37 8.22 55 7.63 Subject 401 27 18.67 28 18.58 37 16.12 55 15.75 Subject 343 27 11.57 28 11.32 37 17.85 55 81 Appendix 2 (Mean Facial Skin Temperature Data) Table A2.1. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 27?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 33.50 35.21 34.47 33.16 33.93 33.61 34.83 33.82 32.70 34.32 33.95 33.57 35.04 34.53 32.74 33.29 33.74 34.81 34.04 32.80 34.11 33.87 33.54 35.06 34.61 32.68 33.18 33.74 34.75 34.06 32.80 34.12 33.85 33.52 35.07 34.72 32.66 33.05 33.73 34.72 34.06 32.82 34.14 33.85 33.40 35.11 34.89 32.59 32.98 33.73 34.71 34.06 32.92 34.15 33.85 33.36 35.13 35.08 32.52 32.91 33.72 34.72 34.07 33.01 34.33 33.88 33.39 35.15 35.23 32.49 32.94 33.72 34.73 34.18 33.14 34.50 33.95 33.46 35.22 35.39 32.62 32.92 33.78 34.74 34.30 33.31 34.72 34.05 33.65 35.26 35.51 32.37 32.90 33.82 34.79 34.35 33.55 34.98 34.12 33.88 35.28 35.57 32.28 32.85 33.93 34.83 34.38 33.72 35.17 34.19 34.17 35.28 35.69 32.28 32.83 34.01 34.84 34.47 33.82 35.29 34.27 34.47 35.30 35.69 32.28 32.83 34.06 34.88 34.54 33.93 35.34 34.33 34.78 35.30 35.92 32.39 32.82 34.21 34.89 34.64 34.03 35.39 34.44 34.99 35.22 35.89 32.47 32.76 34.30 34.92 34.76 34.14 35.44 34.49 35.18 35.17 35.94 32.61 32.73 34.49 34.96 34.87 34.23 35.49 34.57 35.32 35.14 35.96 32.71 32.70 34.63 34.94 34.86 34.35 35.49 34.61 35.44 35.10 35.97 32.53 32.69 34.88 34.97 35.03 34.44 35.53 34.66 35.52 35.18 35.97 32.54 32.65 35.05 35.00 35.14 34.43 35.52 34.70 35.57 35.25 36.02 32.53 32.67 35.19 35.05 35.21 34.50 35.53 34.75 35.60 35.39 36.04 32.59 32.70 35.34 35.08 35.23 34.56 35.56 34.81 35.62 35.44 36.09 32.66 32.75 35.43 34.92 35.27 34.60 35.63 34.84 35.63 35.51 36.13 32.56 32.70 35.45 34.75 35.33 34.70 35.69 34.85 35.66 35.53 36.16 32.54 32.77 35.60 34.61 34.70 35.83 34.82 35.69 35.58 32.58 32.85 35.74 34.52 34.69 35.93 34.70 35.72 35.67 32.64 32.83 35.84 34.46 36.00 34.74 35.76 35.75 32.54 32.88 35.90 34.31 36.07 34.74 35.79 35.83 32.54 32.96 34.30 36.11 34.59 35.86 35.95 32.65 32.95 34.30 36.12 34.64 35.91 35.97 32.64 32.98 34.41 36.21 34.69 35.92 36.03 32.70 33.02 34.36 36.26 34.71 35.96 36.03 32.80 33.09 34.30 34.43 35.87 36.08 32.72 33.27 34.20 34.43 35.87 36.10 32.82 33.31 34.13 34.44 35.97 36.11 32.88 33.43 34.13 34.50 82 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 36.02 36.19 32.98 33.27 34.10 34.51 36.01 36.22 32.98 33.42 34.09 34.55 35.93 36.32 33.00 33.44 34.14 34.57 35.99 36.36 32.96 33.40 34.68 36.06 36.40 33.08 33.52 34.77 36.06 36.44 33.13 33.54 34.79 36.06 36.47 33.21 35.25 35.92 36.48 33.21 35.20 35.95 36.48 33.27 35.23 35.90 36.51 33.35 35.25 35.98 36.57 33.47 35.34 35.96 36.64 33.55 35.38 35.99 36.65 36.32 35.75 36.69 36.22 35.66 36.70 36.18 36.71 36.71 36.71 36.71 36.71 36.71 36.72 36.72 36.73 36.73 36.78 36.78 36.82 36.82 36.83 36.83 36.86 36.86 36.89 36.89 36.91 36.91 36.96 36.96 36.95 36.95 37.00 37.00 36.96 36.96 Table A2.2. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 28?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 32.50 34.90 33.94 32.49 34.23 34.39 34.81 33.79 32.97 34.05 33.81 32.64 34.93 33.73 32.70 34.01 34.13 34.82 34.00 32.85 34.10 33.79 32.63 34.94 33.65 32.66 33.90 34.03 34.80 34.03 32.92 34.10 33.76 32.58 34.92 33.58 32.62 33.79 33.92 34.81 34.08 32.95 34.09 33.73 32.53 34.91 33.55 32.61 33.70 33.81 34.81 34.11 32.97 34.11 33.71 32.49 34.92 33.43 32.56 33.55 33.76 34.81 34.14 32.99 34.22 33.69 32.47 34.97 33.64 32.48 33.40 33.72 34.81 34.26 33.12 34.47 33.73 32.55 34.99 33.75 32.49 33.44 33.75 34.79 34.38 33.36 34.70 33.82 32.71 35.05 33.81 32.44 33.33 33.80 34.78 34.55 33.83 34.85 33.91 33.07 35.12 34.07 32.30 33.27 33.88 34.74 34.66 34.04 35.03 34.02 83 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 33.36 35.17 34.24 32.26 33.24 33.99 34.73 34.80 34.24 35.19 34.12 33.62 35.19 34.52 32.20 33.22 34.15 34.72 34.93 34.29 35.27 34.21 33.89 35.18 34.40 32.39 33.26 34.32 34.74 34.97 34.38 35.33 34.28 34.10 35.18 34.60 32.45 33.37 34.44 34.74 35.02 34.54 35.40 34.39 34.30 35.27 34.69 32.59 33.43 34.58 34.76 35.11 34.65 35.49 34.49 34.48 35.29 34.73 32.80 33.39 34.71 34.77 35.15 34.72 35.57 34.56 34.65 35.33 34.69 32.91 33.58 34.84 34.82 34.69 35.63 34.57 34.85 35.37 34.72 32.71 33.64 34.89 34.85 34.73 35.69 34.60 35.00 35.38 34.87 33.05 33.64 35.03 34.88 34.83 35.76 34.71 35.07 35.34 34.95 33.16 33.72 35.16 34.91 34.87 35.86 34.78 35.23 35.38 34.95 33.08 33.81 35.33 34.94 34.93 35.98 34.85 35.35 35.48 35.13 33.03 33.80 35.46 34.97 35.01 36.06 34.92 35.42 35.48 35.06 33.14 33.77 35.64 35.01 34.79 35.48 35.49 33.21 33.81 35.75 35.01 34.79 35.57 35.56 33.27 33.86 35.88 35.00 34.86 35.59 35.58 33.27 33.88 35.92 34.92 34.86 35.67 35.60 33.23 33.94 36.07 34.76 34.88 35.76 35.62 33.38 33.99 36.14 34.89 34.96 35.77 35.62 33.36 34.06 34.95 34.75 35.78 35.61 33.24 34.21 34.73 34.72 35.79 35.63 33.33 34.30 34.62 34.73 35.84 35.67 33.40 34.46 34.55 34.78 35.88 35.69 33.58 34.50 34.52 34.83 35.93 35.70 33.45 34.51 34.55 34.83 35.95 35.74 33.34 34.57 34.53 34.82 35.94 35.78 33.40 34.64 34.82 34.92 35.89 35.79 33.57 34.58 34.84 34.94 35.85 35.83 33.58 34.69 34.88 34.97 35.86 35.86 33.52 34.70 34.99 35.89 35.85 33.59 34.80 35.03 35.96 35.78 33.67 34.79 35.05 35.99 35.78 33.82 34.92 35.13 36.05 35.79 33.65 34.84 35.08 36.13 35.83 33.87 34.85 35.17 36.18 35.77 33.85 34.85 35.16 36.21 35.78 33.85 34.86 35.18 36.23 35.74 33.89 34.93 35.20 36.25 35.87 33.72 35.28 36.26 35.90 33.76 35.31 36.25 35.96 33.90 35.37 36.25 35.95 33.95 35.38 36.24 35.97 34.04 35.42 36.26 35.92 34.19 35.46 36.29 36.00 36.15 36.35 36.09 36.22 36.34 36.09 36.22 36.36 36.11 36.23 36.38 36.18 36.28 84 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 36.40 36.20 36.30 36.39 36.18 36.28 36.39 36.39 36.35 36.35 36.32 36.32 36.36 36.36 36.32 36.32 36.37 36.37 36.41 36.41 Table A2.3. Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 37?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 32.66 34.81 33.13 34.47 33.81 34.01 34.47 33.59 33.66 34.05 33.87 32.81 34.82 34.59 33.60 34.15 34.46 33.81 33.69 34.17 34.01 32.81 34.90 33.14 34.47 33.50 34.10 34.47 33.90 33.69 34.20 33.92 32.80 34.97 33.03 34.43 33.41 34.06 34.52 34.02 33.74 34.21 33.92 32.77 35.08 33.40 34.40 33.30 33.98 34.54 34.20 33.77 34.18 33.96 32.75 35.17 33.78 34.32 33.19 33.94 34.54 34.43 33.75 34.22 34.01 32.73 35.29 33.98 34.27 33.05 33.85 34.57 34.61 33.81 34.28 34.05 32.72 35.36 34.10 34.27 33.02 33.70 34.60 34.85 33.97 34.52 34.11 32.87 35.46 34.31 34.26 32.90 33.67 34.62 35.04 34.11 34.78 34.20 33.03 35.55 34.55 34.27 32.78 33.70 34.61 35.12 34.31 35.11 34.30 33.31 35.60 34.76 34.52 32.73 33.81 34.57 35.22 34.48 35.33 34.43 33.63 35.65 34.73 34.74 32.72 33.90 34.55 35.35 34.61 35.57 34.54 33.92 35.71 34.83 34.94 32.72 34.08 34.57 35.43 34.81 35.65 34.67 34.16 35.76 35.00 35.03 32.71 34.32 34.59 35.51 34.87 35.71 34.77 34.42 35.77 34.99 35.22 32.83 34.59 34.61 35.61 34.95 35.79 34.88 34.61 35.78 34.92 35.27 32.92 34.78 34.57 35.66 35.06 35.82 34.94 34.79 35.79 35.15 35.30 32.99 34.94 34.58 35.74 35.11 35.84 35.02 34.85 35.87 35.15 35.31 33.06 35.10 34.53 35.17 35.84 34.99 34.99 35.94 35.13 35.34 33.15 35.19 34.53 35.23 35.87 35.04 35.17 36.02 35.20 35.36 33.27 35.37 34.47 35.29 35.90 35.12 35.34 36.11 35.45 35.41 33.34 35.49 34.31 35.31 35.93 35.19 35.42 36.17 35.08 35.45 33.40 35.56 34.26 35.98 35.17 35.58 36.24 34.91 35.52 33.45 35.65 34.42 36.07 35.23 35.66 36.28 35.09 35.60 33.52 35.70 34.43 36.21 35.31 35.72 36.33 35.20 35.66 35.75 34.42 36.35 35.63 35.74 36.35 35.21 35.79 35.86 34.33 36.50 35.68 35.81 36.39 35.20 35.78 35.95 34.24 36.56 35.70 36.16 36.40 35.11 35.86 36.03 34.28 36.62 35.78 36.09 36.42 35.33 35.89 36.16 34.27 35.69 36.43 35.69 35.94 34.20 35.56 36.44 35.73 35.99 34.06 35.56 36.47 35.73 35.81 34.06 35.52 36.49 35.68 35.87 34.08 35.53 36.53 35.75 36.00 36.09 85 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 36.57 35.61 36.14 36.11 36.61 35.43 36.02 36.62 36.62 36.45 36.45 Mean facial skin temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 55?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 33.39 34.77 34.33 34.46 34.42 34.43 34.31 34.37 33.61 33.92 34.20 33.61 34.93 34.37 34.50 34.42 34.50 34.36 34.39 33.69 34.15 34.29 33.56 34.94 34.41 34.47 34.35 34.47 34.36 34.33 33.81 34.17 34.29 33.44 34.95 34.47 34.44 34.31 34.42 34.34 34.30 33.91 34.17 34.27 33.28 34.97 34.41 34.41 34.27 34.38 34.36 34.27 34.09 34.34 34.28 33.12 35.00 34.58 34.41 34.26 34.34 34.32 34.25 34.23 34.59 34.31 32.94 35.01 34.75 34.42 34.20 34.26 34.32 34.19 34.53 34.83 34.34 32.88 35.00 34.74 34.40 34.13 34.30 34.30 34.16 34.81 35.12 34.38 32.89 35.03 34.91 34.36 33.98 34.24 34.29 34.04 34.92 35.35 34.40 33.00 35.06 34.97 34.31 33.92 34.34 34.26 34.02 35.10 35.49 34.45 33.04 35.14 35.05 34.26 33.91 34.46 34.28 34.03 35.20 35.56 34.49 33.26 35.13 35.00 34.15 33.91 34.52 34.35 34.06 35.31 35.65 34.53 33.52 35.09 35.04 34.19 33.95 34.59 34.41 34.10 35.45 35.74 34.61 33.82 35.11 34.26 33.99 34.71 34.46 34.16 35.52 35.85 34.65 34.14 35.14 34.33 34.05 35.06 34.51 34.27 35.57 35.94 34.78 34.37 35.17 34.44 34.13 35.27 34.55 34.36 35.66 35.98 34.88 34.58 35.25 34.54 34.19 35.47 34.55 35.71 36.08 35.05 34.68 35.33 34.50 34.23 35.62 34.51 35.76 36.10 35.09 34.78 35.40 34.54 34.30 35.76 34.38 35.82 36.16 35.14 34.74 35.46 34.45 34.35 35.90 34.36 35.90 36.19 35.17 34.83 34.36 34.42 36.00 34.32 35.96 36.28 35.17 34.22 34.39 36.12 34.26 36.06 36.34 35.23 34.20 34.44 36.17 34.27 36.36 35.09 34.51 36.25 34.28 36.46 35.38 34.56 36.32 34.29 36.48 35.41 34.63 36.48 34.31 36.54 35.49 34.68 34.29 36.62 35.19 34.77 34.28 34.53 34.82 34.34 34.58 34.93 34.44 34.68 34.99 34.47 34.73 35.08 34.48 34.78 35.15 35.15 35.22 35.22 35.33 35.33 35.37 35.37 35.45 35.45 35.55 35.55 35.67 35.67 86 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 35.70 35.70 35.77 35.77 87 Appendix 3 (Acceptability Data) Table A3.1. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 27?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 80 100 80 50 70 80 60 60 80 60 72 40 60 60 50 60 60 40 50 80 50 55 50 20 60 50 60 20 40 40 70 50 46 50 0 60 40 60 20 30 30 70 50 41 40 0 30 40 60 10 20 20 60 40 32 30 0 10 40 40 0 10 10 60 40 24 20 0 20 40 30 0 0 10 60 40 22 20 0 30 20 0 40 18 20 0 30 10 0 12 20 0 30 10 0 12 20 0 30 10 17 10 0 20 10 10 0 20 10 0 20 20 0 0 0 Table A3.2. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 28?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 80 80 80 60 60 100 60 70 90 70 75 60 60 80 50 40 60 60 60 80 60 61 60 20 60 50 40 40 60 40 60 50 48 60 0 50 50 40 20 40 30 60 40 39 60 0 30 50 30 20 40 20 50 20 32 60 0 30 50 20 0 40 50 10 29 60 0 50 20 0 20 0 21 50 0 40 20 0 22 40 0 40 10 0 18 40 0 30 10 0 16 40 0 30 0 18 40 0 20 0 20 40 0 10 0 17 30 0 10 13 20 0 10 88 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 20 20 20 20 20 20 Table A3.3. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 37?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 80 80 100 50 60 90 60 70 90 70 75 60 40 60 50 40 60 40 40 70 60 52 60 20 50 40 40 40 40 20 60 40 41 60 20 50 40 40 20 20 10 60 40 36 60 0 50 30 30 0 10 10 50 30 24 50 0 50 20 20 0 0 60 20 24 40 0 50 20 20 0 0 0 16 30 0 30 20 10 0 0 0 13 0 30 0 10 0 8 0 10 5 Table A3.4. Acceptability (%) data (recorded every 2 minutes) for each subject at a heat index of 55?C Subject Number 001 145 343 358 359 379 401 40 5 419 420 Average 70 80 100 50 60 80 60 60 70 60 69 10 60 100 50 40 40 40 40 60 50 49 0 0 30 50 40 20 40 20 50 40 29 0 0 50 40 10 40 10 50 30 26 0 40 40 0 40 0 60 20 25 0 40 20 0 20 60 10 21 40 20 0 0 0 12 0 0 0 0 0 0 0 Table A3.5. Acceptability (%)for each heat index (?C) condition over time (min) calculated from Equation 5-5. Time A(27) A(28) A(37) A(55) 0 70.89 74.45 72.99 61.39 0.5 67.29 70.71 68.19 57.37 1.5 60.63 63.79 59.50 50.10 2.5 54.63 57.54 51.93 43.75 89 Time A(27) A(28) A(37) A(55) 3.5 49.23 51.91 45.31 38.21 4.5 44.36 46.83 39.54 33.37 5.5 39.97 42.24 34.51 29.14 6.5 36.01 38.10 30.12 25.45 7.5 32.45 34.37 26.28 22.23 8.5 29.24 31.01 22.93 19.41 9.5 26.34 27.97 20.01 16.95 10.5 23.74 25.23 17.47 14.80 11.5 21.39 22.76 15.24 12.93 12.5 19.27 20.53 13.30 11.29 13.5 17.36 18.52 11.61 9.86 14.5 15.65 16.71 10.13 8.61 15.5 14.10 15.07 8.84 7.52 16.5 12.70 13.60 7.71 6.57 17.5 11.45 12.27 6.73 5.73 18.5 10.31 11.06 5.87 5.01 19.5 9.29 9.98 5.13 4.37 20.5 8.37 9.00 4.47 3.82 21.5 7.54 8.12 3.90 3.34 22.5 6.80 7.33 3.41 2.91 23.5 6.13 6.61 2.97 2.54 24.5 5.52 5.96 2.59 2.22 25.5 4.97 5.38 2.26 1.94 26.5 4.48 4.85 1.98 1.69 27.5 4.04 4.38 1.72 1.48 28.5 3.64 3.95 1.50 1.29 29.5 3.28 3.56 1.31 1.13 30.5 2.95 3.21 1.15 0.99 31.5 2.66 2.90 1.00 0.86 32.5 2.40 2.61 0.87 0.75 33.5 2.16 2.36 0.76 0.66 34.5 1.95 2.13 0.66 0.57 35.5 1.75 1.92 0.58 0.50 36.5 1.58 1.73 0.51 0.44 37.5 1.42 1.56 0.44 0.38 38.5 1.28 1.41 0.39 0.33 39.5 1.16 1.27 0.34 0.29 40.5 1.04 1.15 0.29 0.25 41.5 0.94 1.03 0.26 0.22 42.5 0.85 0.93 0.22 0.19 43.5 0.76 0.84 0.20 0.17 44.5 0.69 0.76 0.17 0.15 45.5 0.62 0.68 0.15 0.13 46.5 0.56 0.62 0.13 0.11 47.5 0.50 0.56 0.11 0.10 90 Time A(27) A(28) A(37) A(55) 48.5 0.45 0.50 0.10 0.09 49.5 0.41 0.45 0.09 0.08 50.5 0.37 0.41 0.08 0.07 51.5 0.33 0.37 0.07 0.06 52.5 0.30 0.33 0.06 0.05 53.5 0.27 0.30 0.05 0.04 54.5 0.24 0.27 0.04 0.04 55.5 0.22 0.24 0.04 0.03 56.5 0.20 0.22 0.03 0.03 57.5 0.18 0.20 0.03 0.03 58.5 0.16 0.18 0.03 0.02 59.5 0.14 0.16 0.02 0.02 60.5 0.13 0.15 0.02 0.02 61.5 0.12 0.13 0.02 0.01 62.5 0.11 0.12 0.01 0.01 63.5 0.09 0.11 0.01 0.01 64.5 0.09 0.10 0.01 0.01 65.5 0.08 0.09 0.01 0.01 66.5 0.07 0.08 0.01 0.01 67.5 0.06 0.07 0.01 0.01 68.5 0.06 0.06 0.01 0.01 69.5 0.05 0.06 0.01 0.01 70.5 0.05 0.05 0.00 0.00 71.5 0.04 0.05 0.00 0.00 72.5 0.04 0.04 0.00 0.00 73.5 0.03 0.04 0.00 0.00 74.5 0.03 0.03 0.00 0.00 75.5 0.03 0.03 0.00 0.00 76.5 0.02 0.03 0.00 0.00 77.5 0.02 0.03 0.00 0.00 78.5 0.02 0.02 0.00 0.00 79.5 0.02 0.02 0.00 0.00 80.5 0.02 0.02 0.00 0.00 81.5 0.01 0.02 0.00 0.00 82.5 0.01 0.02 0.00 0.00 83.5 0.01 0.01 0.00 0.00 84.5 0.01 0.01 0.00 0.00 85.5 0.01 0.01 0.00 0.00 86.5 0.01 0.01 0.00 0.00 87.5 0.01 0.01 0.00 0.00 88.5 0.01 0.01 0.00 0.00 89.5 0.01 0.01 0.00 0.00 90.5 0.01 0.01 0.00 0.00 91.5 0.01 0.01 0.00 0.00 92.5 0.00 0.01 0.00 0.00 91 Time A(27) A(28) A(37) A(55) 93.5 0.00 0.00 0.00 0.00 94.5 0.00 0.00 0.00 0.00 95.5 0.00 0.00 0.00 0.00 96.5 0.00 0.00 0.00 0.00 97.5 0.00 0.00 0.00 0.00 98.5 0.00 0.00 0.00 0.00 99.5 0.00 0.00 0.00 0.00 0 10 20 30 40 50 60 70 80 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Time (min) A c c e p t a b i l i t y ( % ) HI=27 HI=28 HI=37 HI=55 Figure A3.1. Average acceptability (%) vs. time (min) for all heat index conditions. Data taken from Table A3.5. Table A3.6. Time (min) to reach acceptability levels (30%, 40%, and 50%) at various heat indices calculated from Equation 5-6 Heat Index (?C) 30% 40% 50% 27 8.20 5.34 3.47 28 8.06 5.22 3.38 29 7.93 5.10 3.28 30 7.80 4.99 3.19 31 7.67 4.88 3.10 32 7.54 4.77 3.02 33 7.41 4.66 2.94 34 7.29 4.56 2.85 35 7.17 4.46 2.78 36 7.05 4.36 2.70 37 6.93 4.26 2.62 38 6.82 4.17 2.55 39 6.70 4.08 2.48 40 6.59 3.99 2.41 41 6.48 3.90 2.35 92 Heat Index (?C) 30% 40% 50% 42 6.37 3.81 2.28 43 6.27 3.73 2.22 44 6.16 3.65 2.16 45 6.06 3.57 2.10 46 5.96 3.49 2.04 47 5.86 3.41 1.98 48 5.76 3.33 1.93 49 5.67 3.26 1.88 50 5.57 3.19 1.82 51 5.48 3.12 1.77 52 5.39 3.05 1.72 53 5.30 2.98 1.68 54 5.21 2.91 1.63 55 5.12 2.85 1.59 93 Appendix 4 (Rectal Temperature Data) Table A4.1. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 27?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.16 37.62 37.7 37.4 37.4 37.31 37.21 37.49 37.29 37.69 37.423 37.17 37.63 37.7 37.4 37.4 37.31 37.24 37.52 37.34 37.7 37.44 37.19 37.64 37.7 37.4 37.4 37.31 37.26 37.54 37.36 37.72 37.451 37.21 37.64 37.7 37.4 37.4 37.31 37.28 37.56 37.38 37.74 37.462 37.26 37.65 37.7 37.4 37.4 37.32 37.31 37.6 37.4 37.76 37.483 37.26 37.66 37.7 37.4 37.4 37.33 37.33 37.62 37.42 37.78 37.498 37.3 37.67 37.8 37.5 37.4 37.36 37.36 37.66 37.45 37.82 37.526 37.32 37.68 37.8 37.5 37.5 37.38 37.39 37.69 37.47 37.84 37.548 37.36 37.7 37.8 37.5 37.5 37.4 37.43 37.74 37.5 37.88 37.578 37.39 37.71 37.8 37.5 37.5 37.44 37.48 37.78 37.54 37.89 37.611 37.43 37.72 37.9 37.5 37.5 37.46 37.51 37.83 37.57 37.92 37.637 37.45 37.75 37.9 37.6 37.6 37.49 37.56 37.87 37.6 37.93 37.662 37.48 37.78 37.9 37.6 37.6 37.52 37.57 37.92 37.63 37.96 37.695 37.51 37.81 37.9 37.6 37.6 37.55 37.61 37.97 37.66 37.98 37.726 37.54 37.82 38 37.7 37.7 37.59 37.65 38.01 37.71 38.01 37.76 37.57 37.85 38 37.7 37.7 37.62 37.68 38.07 37.76 38.02 37.792 37.59 37.88 38 37.7 37.7 37.66 37.69 38.1 37.81 38.06 37.821 37.63 37.89 38 37.7 37.7 37.69 37.7 38.15 37.84 38.11 37.849 37.63 37.91 38 37.8 37.8 37.73 37.72 38.19 37.87 38.12 37.873 37.67 37.93 38.1 37.8 37.8 37.78 37.72 38.24 37.89 38.16 37.905 37.68 37.95 38.1 37.8 37.9 37.81 37.73 38.29 37.94 38.19 37.936 37.72 37.97 38.1 37.8 37.9 37.85 37.74 38.33 37.98 38.22 37.965 37.74 37.99 38.2 37.8 37.9 37.89 37.77 38.02 38.26 37.79 38.01 37.8 37.9 37.93 37.82 38.05 38.29 37.82 38.04 37.9 38 37.97 37.84 38.32 37.84 38.06 37.9 38 37.99 37.87 38.34 37.87 38.07 37.9 38 37.91 38.37 37.89 38.08 37.9 38.1 37.93 38.39 37.9 38.1 37.9 38.1 37.96 38.41 37.92 38.12 37.9 38.1 37.98 38.43 37.92 38.15 38 38.1 37.99 37.92 38.17 38 38.1 38.02 37.92 38.21 38 38.2 38.04 37.92 38.22 38 38.2 38.07 94 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.92 38.24 38 38.2 38.09 37.96 38.27 38.1 38.2 38.11 37.96 38.29 38.1 38.2 38.13 37.98 38.32 38.1 38.2 37.98 38.34 38.1 38.3 37.99 38.35 38.1 38.3 38 38.37 38.2 38.01 38.38 38.2 38.01 38.39 38.2 38.02 38.44 38.2 38.04 38.44 38.2 38.12 38.46 38.2 38.31 38.47 38.3 38.64 38.5 38.3 38.74 38.51 38.3 38.54 38.3 38.57 38.3 38.57 38.59 38.62 38.63 38.66 38.68 38.69 38.73 38.74 38.76 38.79 38.82 38.84 Table A4.2. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 28?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.67 37.63 37.41 37.5 37.37 37.32 37.43 37.45 37.32 37.23 37.433 37.68 37.67 37.42 37.53 37.4 37.34 37.43 37.48 37.32 37.25 37.452 37.68 37.67 37.42 37.53 37.37 37.33 37.44 37.49 37.34 37.26 37.453 37.68 37.67 37.44 37.53 37.39 37.35 37.46 37.51 37.35 37.28 37.466 95 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.67 37.67 37.46 37.54 37.41 37.37 37.46 37.53 37.36 37.29 37.476 37.67 37.69 37.49 37.55 37.43 37.38 37.49 37.55 37.37 37.3 37.492 37.7 37.69 37.54 37.57 37.43 37.41 37.51 37.57 37.4 37.31 37.513 37.77 37.7 37.56 37.59 37.46 37.43 37.52 37.58 37.42 37.33 37.536 37.83 37.71 37.58 37.6 37.47 37.47 37.54 37.61 37.45 37.35 37.561 37.87 37.73 37.61 37.64 37.49 37.5 37.57 37.64 37.47 37.36 37.588 37.88 37.74 37.64 37.66 37.51 37.53 37.59 37.66 37.48 37.37 37.606 37.89 37.74 37.66 37.7 37.52 37.55 37.61 37.69 37.52 37.39 37.627 37.91 37.75 37.7 37.72 37.53 37.6 37.63 37.72 37.54 37.42 37.652 37.96 37.76 37.73 37.75 37.57 37.63 37.68 37.74 37.58 37.43 37.683 37.98 37.77 37.73 37.78 37.58 37.66 37.7 37.78 37.61 37.44 37.703 38 37.78 37.79 37.82 37.59 37.7 37.72 37.81 37.64 37.47 37.732 38.03 37.79 37.81 37.84 37.62 37.73 37.74 37.68 37.48 38.07 37.81 37.84 37.87 37.66 37.77 37.77 37.72 37.49 38.12 37.82 37.88 37.9 37.66 37.81 37.79 37.75 37.52 38.16 37.83 37.9 37.93 37.69 37.85 37.83 37.78 37.54 38.21 37.84 37.94 37.94 37.7 37.9 37.84 37.82 37.57 38.28 37.87 37.96 37.98 37.72 37.94 37.87 37.85 37.58 38.29 37.88 38 37.99 37.75 37.97 37.9 37.61 38.3 37.89 38.02 37.78 38.02 37.93 37.64 38.3 37.91 38.04 37.78 38.05 37.93 37.66 38.3 37.93 38.05 37.82 38.08 37.94 37.68 38.25 37.94 38.07 37.85 38.15 37.94 38.18 37.96 38.08 37.86 38.18 37.94 38.01 37.99 38.09 37.87 37.94 38.01 38.12 37.91 37.94 38.02 38.13 37.93 37.93 38.05 38.14 37.98 38.07 38.17 38 38.09 38.18 38.03 38.11 38.21 38.06 38.13 38.22 38.08 38.14 38.23 38.12 38.16 38.24 38.14 38.18 38.27 38.18 38.19 38.28 38.19 38.22 38.29 38.25 38.25 38.32 38.29 38.27 38.33 38.31 96 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 38.29 38.35 38.33 38.32 38.36 38.35 38.34 38.38 38.39 38.36 38.4 38.43 38.39 38.43 38.41 38.44 38.43 38.46 38.45 38.47 38.47 38.51 38.5 38.53 38.53 38.56 38.58 38.62 38.65 38.66 38.68 Table A4.3. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 37?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.56 37.55 37.63 37.55 37.38 37.24 37.2 37.6 37.18 36.4 37.329 37.58 37.58 37.63 37.66 37.43 37.34 37.2 37.61 37.24 36.5 37.38 37.64 37.59 37.63 37.67 37.44 37.24 37.3 37.61 37.25 36.5 37.381 37.72 37.6 37.66 37.67 37.46 37.26 37.3 37.62 37.27 36.5 37.399 37.78 37.61 37.67 37.68 37.46 37.28 37.3 37.62 37.27 36.5 37.413 37.79 37.63 37.69 37.69 37.48 37.29 37.3 37.63 37.29 36.5 37.427 37.81 37.64 37.76 37.73 37.49 37.31 37.3 37.64 37.33 36.5 37.453 37.84 37.66 37.77 37.75 37.5 37.36 37.4 37.66 37.32 36.5 37.473 37.87 37.67 37.81 37.78 37.53 37.38 37.4 37.66 37.35 36.5 37.499 37.9 37.69 37.86 37.79 37.55 37.42 37.5 37.68 37.38 36.6 37.528 37.93 37.71 37.89 37.82 37.57 37.45 37.5 37.69 37.41 36.6 37.553 37.96 37.73 37.92 37.83 37.58 37.49 37.5 37.71 37.43 36.6 37.577 38.01 37.76 37.98 37.84 37.6 37.51 37.6 37.73 37.5 36.6 37.611 38.06 37.78 37.99 37.85 37.64 37.54 37.6 37.74 37.52 36.6 37.636 37.81 38.02 37.87 37.65 37.57 37.7 37.77 37.56 36.7 37.82 38.04 37.88 37.69 37.63 37.7 37.78 37.59 36.7 37.84 38.07 37.91 37.71 37.63 37.7 37.8 37.62 36.7 97 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.87 38.09 37.93 37.73 37.66 37.7 37.67 36.7 37.88 38.12 37.94 37.77 37.67 37.8 37.71 36.7 37.9 38.14 37.96 37.79 37.71 37.8 37.75 36.7 37.93 38.17 37.97 37.82 37.75 37.8 37.78 36.7 37.96 38.19 38 37.84 37.77 37.8 36.7 37.97 38.21 38.02 37.87 37.81 37.9 36.7 37.99 38.24 38.04 37.91 37.83 37.9 36.7 38.01 38.26 38.06 37.94 37.87 37.9 36.8 38.02 38.28 38.08 37.97 37.95 37.9 36.8 38.03 38.3 38.09 37.99 37.99 38 36.8 38.04 38.32 38.12 38.03 38.03 38 36.8 38.07 38.34 38.14 38.07 38.11 38 38.09 38.37 38.16 38.09 38 38.1 38.39 38.18 38.13 38 38.12 38.41 38.19 38.16 38 38.15 38.45 38.22 38.18 38.1 38.17 38.48 38.25 38.23 38.19 38.27 38.26 38.24 38.28 38.24 38.33 38.24 38.36 38.4 38.43 38.46 38.51 38.54 TableA4.4. Rectal temperature in ?C (recorded every 30 seconds) for each subject at a heat index of 55?C Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.36 37.26 37.58 37.12 37.57 37.41 37.48 37.34 37.28 37.38 37.38 37.26 37.59 37.12 37.57 37.41 37.49 37.36 37.36 37.39 37.39 37.26 37.59 37.13 37.58 37.42 37.51 37.39 37.37 37.40 37.41 37.26 37.59 37.14 37.6 37.44 37.56 37.42 37.39 37.42 37.43 37.26 37.59 37.14 37.61 37.45 37.59 37.46 37.46 37.44 37.46 37.26 37.61 37.16 37.63 37.48 37.61 37.48 37.49 37.46 37.46 37.28 37.62 37.18 37.64 37.5 37.63 37.52 37.52 37.48 37.48 37.31 37.63 37.23 37.66 37.54 37.66 37.54 37.55 37.51 98 Subject Number 001 145 343 358 359 379 401 405 419 420 Average 37.49 37.33 37.64 37.21 37.69 37.57 37.7 37.58 37.59 37.53 37.51 37.36 37.67 37.21 37.7 37.61 37.73 37.61 37.61 37.56 37.53 37.38 37.69 37.22 37.71 37.64 37.77 37.64 37.63 37.58 37.53 37.39 37.73 37.23 37.73 37.67 37.81 37.67 37.64 37.60 37.55 37.41 37.75 37.24 37.76 37.7 37.84 37.7 37.66 37.62 37.43 37.78 37.25 37.78 37.73 37.89 37.74 37.68 37.46 37.82 37.28 37.79 37.75 37.92 37.77 37.69 37.49 37.84 37.29 37.81 37.79 37.96 37.82 37.7 37.52 37.88 37.31 37.83 37.81 37.84 37.71 37.55 37.9 37.33 37.85 37.83 37.88 37.75 37.58 37.93 37.36 37.87 37.84 37.93 37.77 37.61 37.95 37.38 37.89 37.86 37.97 37.79 37.97 37.41 37.9 37.89 38 37.81 37.99 37.43 37.93 37.9 38.06 37.83 38.01 37.46 37.94 37.92 37.84 38.02 37.49 37.96 37.93 37.86 38.04 37.53 37.97 37.95 37.89 37.55 38.01 37.97 37.89 37.58 37.98 37.94 37.61 38.01 37.64 38.02 37.67 38.04 37.7 38.06 37.72 38.08 37.76 37.77 37.81 37.84 37.88 37.88 37.91 37.95 37.97 38 99 Appendix 5 (Performance Time ? Heat Index Model) Table A5.1. Performance time (PT) values (min) for each subject at various heat indices (?C) HI PT (001) PT(145) PT(358) PT(359) PT(379) PT(419) PT(420) PT(405) PT(401) PT(343) 27 27.23 31.73 25.76 21.51 13.35 10.83 13.81 9.12 18.34 11.19 28 26.28 30.03 24.98 21.48 13.34 10.81 13.78 9.06 18.23 11.73 29 25.35 28.42 24.23 21.46 13.32 10.79 13.76 8.99 18.11 12.29 30 24.46 26.89 23.50 21.43 13.31 10.76 13.74 8.93 18.00 12.87 31 23.60 25.45 22.79 21.41 13.29 10.74 13.71 8.86 17.88 13.48 32 22.77 24.08 22.10 21.38 13.28 10.72 13.69 8.80 17.77 14.13 33 21.97 22.79 21.43 21.35 13.26 10.69 13.67 8.74 17.65 14.80 34 21.20 21.57 20.79 21.33 13.25 10.67 13.64 8.67 17.54 15.50 35 20.45 20.41 20.16 21.30 13.23 10.65 13.62 8.61 17.43 16.24 36 19.73 19.31 19.55 21.28 13.22 10.62 13.60 8.55 17.32 17.02 37 19.04 18.28 18.96 21.25 13.21 10.60 13.58 8.49 17.21 17.83 38 18.37 17.30 18.39 21.22 13.19 10.58 13.55 8.43 17.10 18.67 39 17.72 16.37 17.83 21.20 13.18 10.56 13.53 8.37 16.99 19.56 40 17.10 15.49 17.29 21.17 13.16 10.53 13.51 8.31 16.88 20.50 41 16.50 14.66 16.77 21.15 13.15 10.51 13.49 8.25 16.77 21.47 42 15.92 13.87 16.26 21.12 13.13 10.49 13.46 8.19 16.66 22.49 43 15.36 13.13 15.77 21.09 13.12 10.46 13.44 8.13 16.56 23.56 44 14.82 12.42 15.30 21.07 13.10 10.44 13.42 8.07 16.45 24.69 45 14.30 11.76 14.84 21.04 13.09 10.42 13.40 8.01 16.35 25.86 46 13.79 11.13 14.39 21.02 13.08 10.40 13.37 7.96 16.24 27.09 47 13.31 10.53 13.95 20.99 13.06 10.38 13.35 7.90 16.14 28.38 48 12.84 9.96 13.53 20.97 13.05 10.35 13.33 7.84 16.04 29.74 49 12.39 9.43 13.12 20.94 13.03 10.33 13.31 7.79 15.93 31.15 50 11.95 8.92 12.73 20.92 13.02 10.31 13.29 7.73 15.83 32.64 51 11.53 8.44 12.34 20.89 13.01 10.29 13.26 7.68 15.73 34.19 52 11.13 7.99 11.97 20.86 12.99 10.26 13.24 7.62 15.63 35.82 53 10.74 7.56 11.61 20.84 12.98 10.24 13.22 7.57 15.53 37.52 54 10.36 7.16 11.26 20.81 12.96 10.22 13.20 7.51 15.43 39.31 55 10.00 6.77 10.92 20.79 12.95 10.20 13.17 7.46 15.33 41.18 100 Appendix 6 (Survey Data) Table A6.1. Perceived Effort and Reward Questionnaire Results Subject Score 359 0.00 405 1.00 419 0.21 420 0.51 343 0.64 379 0.21 145 0.41 401 0.54 001 0.49 358 0.21 Table A6.2. Claustrophobia Questionnaire Results Subject Score 359 0.00 405 0.92 419 0.65 420 0.50 343 0.58 379 0.42 145 0.81 401 1.15 001 1.04 358 0.75 Table A6.3. Physical Activity Questionnaire Results Subject kcal/wk 359 4396 405 5108 419 6316 420 5928 343 22624 379 3262 145 2078 401 3878 001 28616 358 3768 101 Table A6.4. Respirator User Questionnaire Results. Frequency represents the number of times the respirator had been worn prior to taking part in the study. Subject Frequency 359 30 405 6 419 0 420 0 343 25 379 8 145 80 401 17.5 1 80 358 50 Table A6.5. MBTI Personality Type Questionnaire Results EI SN TF JP Subject E I EI S N SN T F TF J P JP 001 4 6 0.40 5 15 0.25 5 5 0.50 16 4 0.80 145 9 1 0.90 6 14 0.30 5 15 0.25 11 9 0.55 358 10 0 1.00 9 11 0.45 7 13 0.35 9 11 0.45 359 4 6 0.40 14 6 0.70 3 17 0.15 13 7 0.65 405 1 9 0.10 10 10 0.50 5 15 0.25 11 9 0.55 419 6 4 0.60 15 5 0.75 15 5 0.75 16 4 0.80 420 9 1 0.90 17 3 0.85 7 13 0.35 7 13 0.35 343 5 5 0.50 10 10 0.50 7 3 0.70 11 9 0.55 379 3 7 0.30 16 4 0.80 11 9 0.55 13 7 0.65 401 3 7 0.30 12 8 0.60 2 18 0.10 12 8 0.60 102 Appendix 7 (C 1 graphs) All C 1 values are from Equation 5-3. 0 20 40 60 80 100 120 140 160 0 10 20 30 40 50 60 70 80 Ve (L/min) C 1 Female Male Figure A7.1. C 1 vs. V e from Table A1.2. for each subject (n=10). 0 20 40 60 80 100 120 140 160 0 0.2 0.4 0.6 0.8 1 1.2 PERQ C 1 Figure A7.2. C 1 vs. Perceived Effort and Reward Questionnaire results from Table A6.1. for each subject (n=10). 103 0 20 40 60 80 100 120 140 160 0 0.2 0.4 0.6 0.8 1 1.2 1.4 CLQ C 1 Figure A7.3. C 1 vs. Claustrophobia Questionnaire results from Table A6.2. for each subject (n=10). 0 20 40 60 80 100 120 140 160 0 5000 10000 15000 20000 25000 30000 35000 Physical Activity (kcal/wk) C 1 Figure A7.4. C 1 vs. level of physical activity results from Table A6.3. for each subject (n=10). 104 0 20 40 60 80 100 120 140 160 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 EI C 1 Figure A7.5. C 1 vs. Extroversion/Introversion personality characteristic from Table A6.5. for each subject (n=10). 0 20 40 60 80 100 120 140 160 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 SN C 1 Figure A7.6. C 1 vs. Sensing/Intuition personality characteristic from Table A6.5. for each subject (n=10). 105 0 20 40 60 80 100 120 140 160 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 TF C 1 Figure A7.7. C 1 vs. Thinking/Feeling personality characteristic from Table A6.5. for each subject (n=10). 0 20 40 60 80 100 120 140 160 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 JP C 1 Figure A7.8. C 1 vs. Judging/Perception personality characteristic from Table A6.5. for each subject (n=10). 106 0 20 40 60 80 100 120 140 160 0 102030405060708090 # of times Respirator worn C 1 Figure A7.9. C 1 vs. Respirator Familiarity from Table A6.4. for each subject (n=10). 107 Appendix 8 (Research Surveys) 108 109 110 111 112 Respirator User Questionnaire Please answer all questions to the best of your ability. A. Using your respirator. 1. For how many years have you been wearing respirators? ________ 2. How many times have you worn a respirator while performing manual labor? ____________ 3. If you have worn a respirator before for how long at a time do you wear a respirator? ______________ B. In this particular study, the respirator became a burden. What were the reasons for terminating the test (circle all that apply)? TOO HOT TOO SWEATY TOO HEAVY TOO TIGHT DIFFICULT SEEING DIFFICULT BREATHING DIFFICULT MOVING DIFFICULT TO DO THE JOB FELT AWKWARD OR CLUMSY FELT SELF-CONSCIOUS FELT ANXIOUS OR CLAUSTROPHOBIC OTHER (please specify) ___________________________ C. Rate your attitude toward respirator masks: Unfavorable Neutral Favorable 1 2 3 4 5 D. Respirators and work. 1. Rate how hard you think the following activities are WITHOUT wearing a respirator mask. (Circle one for each activity). Very easy Moderate Very hard Running to catch a bus 1 2 3 4 5 Machine working, welding 1 2 3 4 5 Writing 1 2 3 4 5 113 Walking through deep snow 1 2 3 4 5 Climbing stairs 1 2 3 4 5 Running fast 1 2 3 4 5 Walking while carrying a heavy load 1 2 3 4 5 Sweeping floors 1 2 3 4 5 Washing clothes 1 2 3 4 5 Shoveling fast 1 2 3 4 5 2. Answer these questions according to how you felt on average during each testing session. WHEN I USE MY RESPIRATOR: Totally disagree Neutral Totally agree The respirator does not interfere with my vision. 1 2 3 4 5 I stay cool. I don?t sweat because of my respirator. 1 2 3 4 5 I can breathe easily. The respirator doesn?t interfere with my breathing. 1 2 3 4 5 The respirator is not heavy. 1 2 3 4 5 The respirator harness straps are comfortable. 1 2 3 4 5 I feel O.K. inside the mask. 1 2 3 4 5 Wearing the respirator does not interfere with my work. 1 2 3 4 5 114 Appendix 9 (Acceptability Scales) RPE 6 7 very, very light 8 9 very light 10 11 fairly light 12 13 somewhat hard 14 15 hard 16 17 very hard 18 19 very, very hard 20 115 BACS 10 very, very comfortable 8 comfortable 6 fairly comfortable 4 fairly uncomfortable 2 uncomfortable 0 very, very uncomfortable 116 FT/OT 1 very cold 2 cold 3 slightly cool 4 neutral 5 slightly warm 6 hot 7 very hot 117 Appendix 10 (IRB 05-0245) 118 119 120 121 122 1. Abstract: Respirators are worn in the workplace in order to protect the individual from toxic airborne substances; however, respirators are worn in only about 20-30% of the appropriate circumstances due to several factors. The primary importance for the proposed research is to study the thermal effects due to increased temperature and humidity of inspired air. An environmental chamber will be utilized for simulation of various ambient conditions, and a heating unit will supply various air conditions to the respirator. Subjects will don a full face piece respirator and exercise at 65-70% of their VO2max at three previously determined conditions. A mathematical model will be developed that correlates performance time with temperature and humidity to provide manufacturers with a useful tool for designing masks with favorable characteristics that are appropriate for optimal performance. 2. Subject Selection: a. Who will the Subjects be? How will you recruit them? If you plan to advertise for subjects, include a copy of the advertisement. The subjects will be students at the University of Maryland. They will be recruited from willing and able volunteers at the university, specifically, within the Department of Biological Resources Engineering. The subjects will receive no course credit for their participation. Twenty total subjects will be recruited via announcements at the conclusion of Biological Resources Engineering class periods. The studies will be conducted at the University of Maryland. b. Will the subjects be selected for any specific characteristics (e.g. age, sex, race, ethnic origin, religion or any social or economic qualifications)? No; however, the subjects will be screened prior to testing when the informed consent document is completed to ensure that they are in good physical health. c. State why the selection will be made on the basis or base given in 2(b). The purpose of the study is to determine comfort level of the respirator, and not whether or not it corrects a specific problem. For this reason, any and all volunteers will be selected. d. How many subjects will participate in this protocol? 15 subjects 3. Procedures Section: What precisely will be done to the subjects? Explain in detail your methods and procedures in terms of what will be done to subjects. How many subjects will be recruited? What is the total investment of time of the subjects. If subjects will complete surveys and/or other Instruments on more than one occasion, state this in the procedures 123 section. If you are using a questionnaire or handout, include a copy within each set of application documents. If you are conducting a focus group, include a list of the questions for the focus group. The research will consist of three stages: obtain consent and orientation, perform the VO2max graded exercise test, and 65-70% VO2max testing under three different conditions. The initial analysis suggests that a total of 20 subjects will be needed for the study. The first stage is to obtain consent and give a brief orientation to the subjects to describe what their participation requires. The subjects may or may not be familiar with wearing a respirator; therefore, each subject will be fully advised as to the requirements of their participation. The subjects will read and sign the informed consent document and fill out a brief medical history questionnaire. This session provides the subject with a detailed description of their rights, and it provides the investigators with information about the subjects'' health and ability to partake in vigorous activity. Any demographic or experimental data collected will remain confidential and correspond only to a subject number. The second stage of research consists of a graded exercise test (VO2max test) that will be used to determine the subject?s maximal aerobic capacity. This test provides valuable information necessary for determining each subject?s work rate during each of the testing sessions, along with information regarding each subject?s critical termination values such as heart rate and maximum oxygen consumption. Participants will be asked to warm-up and stretch for approximately ten minutes prior to the start of the test. The mask used to collect gases during the test is a half face mask equipped with one-way inhalation and exhalation valves. Heart rate measurements will be assessed using a standard ECG electrode configuration with the leads connected to a Patient Monitoring System. In order to determine VO2max, the subject will partake in a graded exercise test to exhaustion. The work rate will be adjusted every three minutes until the participant becomes fatigued, fails to display a rise in oxygen consumption in concurrence with the increase in work rate, or reaches a maximal heart rate. The third stage of research consists of actual subject testing in an environmental chamber. Each session will be conducted at 65-70% of the participant?s maximal oxygen consumption using a treadmill. All sessions will utilize a full face piece mask, which covers the entire face including the cheeks and forehead. The subject will exercise at a constant work rate throughout all three sessions. Furthermore, each of the subjects will dress similarly and wear the same mask throughout all sessions. Before exercise, the subject will complete a five minute warm-up on the treadmill followed by five minutes of stretching. The subject will don a heart rate monitor, rectal probe for monitoring core body temperature, and three surface temperature sensors on the face. The sensors will be placed on the mid-forehead, right cheekbone, and upper lip under the left nostril. The rectal probe will be inserted by the subject, who will be given lubricant to ease in insertion of the rectal probe. The human monitor will explain the insertion technique, which will be to insert the tip gradually into the sphincter approximately the length of the palm of the subject?s hand (approximately 2 inches). The subject will be asked to dress in 124 standard military fatigues and tennis shoes. A full face piece respirator will be fitted to the subject for a comfortable, but snug fit. The outlet of the respirator will be connected via a hose to monitor continuous expired airflow, and the inspiratory valve will be connected to the outlet of the humidity chamber. The subject will begin to exercise at a treadmill speed and grade set at a work rate below 65-70% VO2max; the speed and grade will be increased for approximately 90 seconds before the speed and grade corresponding to 65-70% VO2max is reached. At this work rate, the subject will be asked to exercise until he or she reaches volitional fatigue. A 5 minute cool down period will follow the testing session. A human monitor while be present during subject testing to monitor for signs of distress. Heart rate, facial skin temperature, and core body temperature will be monitored; Rating of Perceived Exertion (RPE), and the Breathing Apparatus Comfort Scales (BACS) will be recorded each minute. The RPE (6-20) and BACS (0-8) scales will be used to assess fatigue and comfort levels of the subject. A high RPE value indicates that the subject has reached exhaustion, and a low BACS value indicates that the subject views the mask conditions as uncomfortable. Both facial thermal and whole body thermal sensation values will be recorded. A participant is free to withdraw from this project at anytime without incurring a penalty. This request may be expressed to an investigator through either verbal or written communication. 4. Risks and Benefits: Are there any risks to the subjects? If so, what are these risks? What are the benefits? If there are known risks associated with the subject's participation in the research, what potential benefits will accrue to justify taking these risks? Possible risks to the subject during the maximum oxygen consumption test are minimal and including tripping, falling off of the treadmill, and heat stress, though these risks are not anticipated. Possible risks to the subject include heat stress, severe exhaustion, or injury due to falling off of the treadmill. A human monitor will be present throughout all testing sessions, and at any time during the testing, if a subject begins to show signs of extreme discomfort, or expresses extreme discomfort, the testing session will be terminated. Due to the possibility of dehydration or heat stress, water will be available for the subject?s use at any time during testing. As protection against heat stroke, core body temperature will be monitored throughout the study. Testing will be terminated if rectal temperature reaches 103?F. High rectal temperature is indicative of heat generated in the active muscles, and if this reaches the critical value, the person may become incapacitated. As a result of normal exercise, core body temperature generally rises to about 101?F; however, heat stroke occurs at core body temperatures of 106?F; therefore, in order to avoid approaching symptoms of heat 125 stroke, the cut-off temperature will be 103?F (McArdle, 1996).Core Body temperature will be monitored through a rectal probe connected to a computerized temperature monitoring system, after dressing has been completed. Insertion of rectal probes could cause some minor discomfort. The tips are round and will not hurt the subject. The rectal probes will be disposed off using biohazard disposal methods used in the laboratories. The study is not designed to help the subjects. Participants will receive a copy of their test results and this information may be of interest to those individuals interested in duplicating optimal environmental conditions. No monetary benefits will be provided. There are no direct benefits to the subjects for their participation in this study. The risks associated with this project are minimal and reversible with adequate rest. The benefits are specific to those persons responsive to heat stimulus and therefore will be of interest to this group. This benefit will not be transferred to other individuals; however, the minimal risk encountered makes this project feasible for this population. 5. Confidentiality Adequate provisions must be made to protect the privacy of subjects and to maintain confidentiality of identifiable information. Explain how our procedures accomplish this objective, including such information as the means of data storage, data location and duration, description of persons with access to the data, and method of destroying the data when completed. If the research involves audiotaping, videotaping or digital recordings, state who will have access to the tapes or recordings, where the tapes or recordings will be kept, and state the final disposition of the tapes or recordings (i.e. Will the tapes or recordings be destroyed? If so, when will the tapes or recordings be destroyed?). The results of each subject will be stored under a number relating only to the order in which they were tested. Subjects willing to be reached for further questions and comments will have a chance to give us their contact information. Statistical information used in reports concerning this project will in no way be linked to any participants of the study. 6. Information and Consent Forms State specifically what information will be provided to the subjects about the investigation. Is any of this information deceptive? State how the subject's informed consent will be obtained. Include a final draft of the consent form that you propose to use. Include a description of the data storage methods which will be used to ensure confidentiality within the consent form. Each subject will read and sign the consent form and questions regarding the study will be answered at this time. Prior to the study, the subject will receive the attached information, including a description of the purpose of the study, the questionnaire, and consent form. Only subjects who have signed the consent forms will be allowed to participate in the study. 7. Conflict of Interests 126 Describe the potential conflict of interest, including how such a conflict would affect the level of risk to the study participants. 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